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In this study, we explored the role of miR\u2010615\u20103p in the osteogenic differentiation of human LF cells. The expression of miR\u2010615\u20103p was detected during the osteogenic differentiation of hFOB1.19 human osteoblasts, human BMSCs, and human LF cells. The qPCR results showed that miR\u2010615\u20103p was being decreased during the osteogenic differentiation of these cell lineages. Then, both gain\u2010 and loss\u2010function experiments, respectively performed by single\u2010strand miR\u2010615\u20103p mimic and antagomir, revealed that miR\u2010615\u20103p negatively regulated the osteogenesis of hLF cells, manifested by a lighter staining degree with Alizarin Red and a decreased level of osteogenic marker genes, including alkaline phosphatase (ALP), RUNX2, osterix (ostx), osteocalcin (OCN), and osteopontin (OPN). Subsequently, our data on bioinformatic analysis, 3\u2032\u2010UTR luciferase activity assay, and protein level detection indicated that miR\u2010615\u20103p directly targeted and suppressed the expression of FOXO1 and GDF5. Furthermore, knockdown of either FOXO1 or GDF5 could inhibit the osteogenic differentiation of hLF cells, which displayed a similar effect with the miR\u2010615\u20103p mimic. In conclusion, miR\u2010615\u20103p negatively regulates the osteogenic differentiation of hLF cells through post\u2010transcriptionally suppressing osteogenic regulators GDF5 and FOXO1. It can be regarded as a potential target for human OLF therapy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10780","type":"journal-article","created":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T23:26:47Z","timestamp":1493681207000},"page":"779-786","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["MiR\u2010615\u20103p inhibits the osteogenic differentiation of human lumbar ligamentum flavum cells via suppression of osteogenic regulators GDF5 and FOXO1"],"prefix":"10.1002","volume":"41","author":[{"given":"Jichao","family":"Yin","sequence":"first","affiliation":[{"name":"Department of Epidemiology and Biostatistics School of Public Health Xi'an Jiaotong University Health Science Center No. 76 West Yanta Road Xi'an Shaanxi 710061 China"},{"name":"Department of Orthopedics and Traumatology Xi'an Hospital of Traditional Chinese Medicine Xi'an 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