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However, its precise role in first\u2010trimester trophoblast cells is still unknown. WNT2 expression in the villous tissues of unexplained recurrent spontaneous abortion (URSA) patients was compared with that of healthy women by Western blot. The function of WNT2 in HTR\u20108\/SVneo trophoblast cells was evaluated by altering the cellular WNT2 level through overexpression and shRNA knockdown. The molecular mechanism of the effect of WNT2 on trophoblast cells was investigated. The association of WNT2 with the Wnt\/\u03b2\u2010catenin signaling pathway was studied through Western blot and immunofluorescence. Results showed that WNT2 protein expression was significantly decreased in villi of the URSA group compared with the control group. In vitro studies showed that WNT2 could promote human trophoblast cell proliferation and migration through activating the Wnt\/\u03b2\u2010catenin signaling pathway. Moreover, upon the knockdown of WNT2, trophoblast cell proliferation and migration were significantly suppressed. In conclusion, our study indicated that WNT2 plays an important role in trophoblast function. WNT2 insufficiency might cause impaired trophoblast cell proliferation and migration via downregulation of Wnt\/\u03b2\u2010catenin signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10807","type":"journal-article","created":{"date-parts":[[2017,6,19]],"date-time":"2017-06-19T10:26:39Z","timestamp":1497867999000},"page":"898-907","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Decreased expression of WNT2 in villi of unexplained recurrent spontaneous abortion patients may cause trophoblast cell dysfunction via downregulated Wnt\/\u03b2\u2010catenin signaling pathway"],"prefix":"10.1002","volume":"41","author":[{"given":"Ning","family":"Li","sequence":"first","affiliation":[{"name":"Central Laboratory the Affiliated Yantai Yuhuangding Hospital of Qingdao University Yantai Shandong 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