{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T02:40:41Z","timestamp":1780368041825,"version":"3.54.1"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,1,24]],"date-time":"2018-01-24T00:00:00Z","timestamp":1516752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004083","name":"Ministry of Science ICT and Future Planning","doi-asserted-by":"publisher","award":["2015R1A2A2A01006120"],"award-info":[{"award-number":["2015R1A2A2A01006120"]}],"id":[{"id":"10.13039\/501100004083","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Tacrolimus is an immunosuppressive drug that inhibits the release of inflammatory cytokines involved in rheumatoid arthritis development by blocking T cell activation. \u201cEndoplasmic reticulum stress,\u201d an imbalance between protein folding load and capacity leading to the accumulation of unfolded proteins in the endoplasmic reticulum lumen, has been implicated in rheumatoid arthritis and other inflammatory and metabolic diseases. We aimed to investigate the effect of tacrolimus on endoplasmic reticulum stress\u2013mediated osteoclastogenesis and inflammation and elucidate the underlying mechanisms. In vitro studies were performed using mouse bone marrow cells that were cultured with or without interleukin\u20101\u03b2, thapsigargin, or tacrolimus to induce osteoclast differentiation. A mouse model of arthritis was established by immunizing mice with bovine type II collagen. Tacrolimus was orally administered to mice from day 20 to 45 following the initial immunization, and histopathological changes and expression of specific biomarkers of endoplasmic reticulum stress\u2013mediated inflammatory signaling pathways were examined. In vitro, tacrolimus inhibited receptor activator of nuclear factor\u2010\u03baB ligand\u2010mediated osteoclast formation augmented by interleukin\u20101\u03b2, thapsigargin, or both. Furthermore, tacrolimus inhibited glucose\u2010regulated protein (GRP78), protein kinase R\u2010like endoplasmic reticulum kinase, inositol\u2010requiring enzyme 1 (IRE 1), and activating transcription factor 6 (ATF6) augmented by interleukin\u20101\u03b2, thapsigargin, or both. Tacrolimus significantly ameliorated osteolysis and endoplasmic reticulum stress intensity in mice. Simultaneously, it reduced inflammatory cell infiltration, osteoclastogenesis, and inflammatory responses by inhibiting GRP78, IRE 1, and ATF6. These findings suggest that tacrolimus exhibits an anti\u2010inflammation effect in rheumatoid arthritis and might inhibit joint damage progression by inhibiting endoplasmic reticulum stress.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10861","type":"journal-article","created":{"date-parts":[[2017,8,22]],"date-time":"2017-08-22T12:22:39Z","timestamp":1503404559000},"page":"393-402","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Tacrolimus regulates endoplasmic reticulum stress\u2013mediated osteoclastogenesis and inflammation: In vitro and collagen\u2010induced arthritis mouse model"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4334-2751","authenticated-orcid":false,"given":"Won\u2010Seok","family":"Lee","sequence":"first","affiliation":[{"name":"Division of Rheumatology, Department of Internal Medicine Research Institute of Clinical Medicine of Chonbuk National University\u2010Biomedical Research Institute of Chonbuk National University Hospital Jeonju South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u2010Hyeon","family":"Jeong","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Internal Medicine Research Institute of Clinical Medicine of Chonbuk National University\u2010Biomedical Research Institute of Chonbuk National University Hospital Jeonju South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun\u2010Gyeong","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Internal Medicine Research Institute of Clinical Medicine of Chonbuk National University\u2010Biomedical Research Institute of Chonbuk National University Hospital Jeonju South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunjung","family":"Choi","sequence":"additional","affiliation":[{"name":"Division of 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