{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T12:08:27Z","timestamp":1793189307886,"version":"4.5.0"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,9,25]],"date-time":"2017-09-25T00:00:00Z","timestamp":1506297600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572140"],"award-info":[{"award-number":["81572140"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Mesenchymal stem cells (MSCs)\u2010derived exosomes exhibit protective effects on damaged or diseased tissues. Hypoxia\u2010inducible factor 1\u03b1 (HIF\u20101\u03b1) plays a critical role in bone development. However, HIF\u20101\u03b1 is easily biodegradable under normoxic conditions. The bone\u2010marrow\u2010derived mesenchymal stem cells (BMSCs) were transfected with adenovirus carrying triple point\u2010mutations (amino acids 402, 564, and 803) in the HIF\u20101\u03b1 coding sequence (CDS). The mutant HIF\u20101\u03b1 can efficiently express functional proteins under normoxic conditions. To date, no study has reported the role of exosomes secreted by mutant HIF\u20101\u03b1 modified BMSCs in the recovery of the early steroid\u2010induced avascular necrosis of femoral head (SANFH). In this study, we firstly analyzed exosomes derived from BMSCs modified by mutant (BMSC\u2010Exos<jats:sup>MU<\/jats:sup>) or wild\u2010type HIF\u20101\u03b1 (BMSC\u2010Exos<jats:sup>WT<\/jats:sup>). In vitro, we investigated the osteogenic differentiation capacity of BMSCs modified by BMSC\u2010Exos<jats:sup>MU<\/jats:sup> or BMSC\u2010Exos<jats:sup>WT<\/jats:sup>, and the angiogenesis effects of BMSC\u2010Exos<jats:sup>MU<\/jats:sup> and BMSC\u2010Exos<jats:sup>WT<\/jats:sup> on human umbilical vein endothelial cells (HUVECs). Besides, the healing of the femoral head was also assessed in vivo. We found that the potential of osteogenic differentiation of BMSCs treated with BMSC\u2010Exos<jats:sup>MU<\/jats:sup> was higher than the wild\u2010type group in vitro. In addition, BMSC\u2010Exos<jats:sup>MU<\/jats:sup> stimulated the proliferation, migration, and tube formation of HUVECs in a dose\u2010dependent manner. Compared with the BMSC\u2010Exos<jats:sup>WT<\/jats:sup> or PBS control group, the injection of BMSC\u2010Exos<jats:sup>MU<\/jats:sup> into the necrosis region markedly accelerated the bone regeneration and angiogenesis, which were indicated by the increased trabecular reconstruction and microvascular density. Taken together, our data suggest that BMSC\u2010Exos<jats:sup>MU<\/jats:sup> facilitates the repair of SANFH by enhancing osteogenesis and angiogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10869","type":"journal-article","created":{"date-parts":[[2017,9,6]],"date-time":"2017-09-06T16:44:41Z","timestamp":1504716281000},"page":"1379-1390","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":104,"title":["Exosomes secreted from mutant\u2010HIF\u20101\u03b1\u2010modified bone\u2010marrow\u2010derived mesenchymal stem cells attenuate early steroid\u2010induced avascular necrosis of femoral head in rabbit"],"prefix":"10.1002","volume":"41","author":[{"given":"Haile","family":"Li","sequence":"first","affiliation":[{"name":"Department of Orthopedics The First Affiliated Hospital of Jinzhou Medical University Jinzhou 121001 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danping","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The First Affiliated Hospital of Jinzhou Medical University Jinzhou 121001 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Li","sequence":"additional","affiliation":[{"name":"Biobank The First Affiliated Hospital of Jinzhou Medical University Jinzhou 121001 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanjian","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The First Affiliated Hospital of Jinzhou Medical University Jinzhou 121001 P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dachuan","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The First Affiliated Hospital of Jinzhou Medical University Jinzhou 121001 P.R. 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