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Our previous data demonstrated that type II cGMP\u2010dependent protein kinase (PKG II) could block the EGF\u2010EGFR axis as well as down\u2010stream signaling pathways, for example, MAPK, PI3\u2009K, and PLC in GC cells. However, the exact mechanisms of PKG II against cancer remain unclear. Therefore, the present work was to address the above question. Human GC cell line AGS was infected with adenoviral construct encoding cDNA of PKG II (Ad\u2010PKG II) to up\u2010regulate PKG II and then treated with 8\u2010pCPT\u2010cGMP. Two\u2010dimensional electrophoresis (2\u2010DE) was used to analyze the changes of protein expression in the cells. The results showed that 17 proteins had more than twofold changes in EGF\u2010treated group compared with control. However, Ad\u2010PKG II could effectively reversed the changes. Furthermore, far upstream element\u2010binding protein 1 (FUBP1) and MarvelD3 were chosen and PKG II activation reversed EGF\/EGFR\u2010induced up\u2010regulation of FUBP1 and downregulation of MarvelD3, respectively. MarvelD3 silence effectively abolished the inhibitory effect of PKG II on EGF\u2010triggered migration. These data indicated that the inhibitory effect of PKG II partially was associated with MarvelD3.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10912","type":"journal-article","created":{"date-parts":[[2017,11,18]],"date-time":"2017-11-18T11:52:36Z","timestamp":1511005956000},"page":"435-442","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["PKG II effectively reversed EGF\u2010induced protein expression alterations in human gastric cancer cell lines"],"prefix":"10.1002","volume":"42","author":[{"given":"Hai","family":"Qian","sequence":"first","affiliation":[{"name":"Medical School Jiangsu University 301 Xuefu Road Zhenjiang Jiangsu 212013 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Tao","sequence":"additional","affiliation":[{"name":"Medical School Jiangsu University 301 Xuefu Road Zhenjiang Jiangsu 212013 P. R. 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