{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T22:08:31Z","timestamp":1782166111692,"version":"3.54.5"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,3,26]],"date-time":"2018-03-26T00:00:00Z","timestamp":1522022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Chinese Medicine Research Project of Heilongjiang Province","award":["No. ZHY16-015"],"award-info":[{"award-number":["No. ZHY16-015"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Inhibition of bone regeneration by wear debris is the main cause of peri\u2010prosthetic osteolysis. Here, we investigated the effect of icariin on cell proliferation, apoptosis, osteogenic differentiation and matrix mineralization of osteoblasts in an in vitro model of titanium (Ti) particle\u2010induced osteolysis. In the present study, MC3T3\u2010E1 cells were pretreated with 10<jats:sup>\u22128<\/jats:sup>\u2009M icariin for 4\u2009h and then incubated with Ti particles (0.1\u2009mg\/mL). The results showed that Ti particles inhibited cell proliferation and promoted cell apoptosis of MC3T3\u2010E1 cells, whereas icariin pretreatment blocked the effect of Ti particles. In addition, we found that icariin stimulation alone increased ALP activity, accelerated matrix mineralization and upregulated the levels of bone morphogenetic protein 2 (BMP2), Runt\u2010related transcription factor 2 (Runx2), osteocalcin (OCN) and miR\u201021\u20105p; whereas, Ti particles alone exerted the opposite effects. Icariin partly reversed the effect of Ti particles on cell differentiation and mineralization. Twenty hours after transfection with antagomiR\u201021\u20105p or antagomiR\u2010NC, the cells were pretreated with icariin for 4\u2009h and then incubated with Ti particles. Further studies showed that partial knockdown of miR\u201021\u20105p abolished the promotion effect of icariin on osteoblast differentiation and matrix mineralization in Ti particle\u2010stimulated MC3T3\u2010E1 cells. In conclusion, miR\u201021\u20105p may be a potential pro\u2010osteogenesis regulator and icariin may protect against Ti particle\u2010induced inhibition of osteogenic differentiation and mineralization through upregulation of miR\u201021\u20105p.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10957","type":"journal-article","created":{"date-parts":[[2018,3,3]],"date-time":"2018-03-03T11:26:40Z","timestamp":1520076400000},"page":"931-939","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Icariin attenuates titanium particle\u2010induced inhibition of osteogenic differentiation and matrix mineralization via miR\u201021\u20105p"],"prefix":"10.1002","volume":"42","author":[{"given":"Feng","family":"Lian","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery The Fourth Affiliated Hospital of Harbin Medical University Harbin 150001 People's Republic of 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