{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T23:17:27Z","timestamp":1786749447354,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,6,15]],"date-time":"2018-06-15T00:00:00Z","timestamp":1529020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LQ14H080002"],"award-info":[{"award-number":["LQ14H080002"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY12H08002"],"award-info":[{"award-number":["LY12H08002"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY16H080006"],"award-info":[{"award-number":["LY16H080006"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The survival rate of childhood acute lymphoblastic leukemia (ALL) has increased while that of Philadelphia\u2010positive (Ph+) ALL remains low. CD19 is a B\u2010cell specific molecule related to the survival and proliferation of normal B cells. However, there is little information available on the effects of CD19 on the biological behavior of Ph+ ALL cells. In this study, we explored a lentiviral vector\u2010mediated short hairpin RNA (shRNA) expression vector to stably reduce CD19 expression in Ph+ ALL cell line SUP\u2010B15 cells and investigated the effects of CD19 downregulation on cell proliferation, apoptosis, drug sensitivity, cell adhesion, cell migration and cell invasion in vitro. CD19 mRNA and protein expression levels were inhibited significantly by CD19 shRNA. Down\u2010regulation of CD19 could inhibit cell proliferation, adhesion, migration and invasion, and increase cell apoptosis and the efficacy of chemotherapeutic agents and imatinib in SUP\u2010B15 cells. Moreover, we found that down\u2010regulation of CD19 expression inhibits cell proliferation and induces apoptosis in SUP\u2010B15 cells in a p53\u2010dependent manner. Taken together, our results suggest that lentiviral vector\u2010mediated RNA interference of CD19 gene may be a promising strategy in the treatment of Ph+ ALL.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10994","type":"journal-article","created":{"date-parts":[[2018,5,29]],"date-time":"2018-05-29T20:13:55Z","timestamp":1527624835000},"page":"1228-1239","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Down\u2010regulation of CD19 expression inhibits proliferation, adhesion, migration and invasion and promotes apoptosis and the efficacy of chemotherapeutic agents and imatinib in SUP\u2010B15 cells"],"prefix":"10.1002","volume":"42","author":[{"given":"Junqing","family":"Wu","sequence":"first","affiliation":[{"name":"Division of Hematology the First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000 PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Liang","sequence":"additional","affiliation":[{"name":"Division of Hematology the First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000 PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Qian","sequence":"additional","affiliation":[{"name":"Division of Pediatric Hematology\u2010Oncology the First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000 PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyuan","family":"Tang","sequence":"additional","affiliation":[{"name":"Division of Hematology the First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000 PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chongyun","family":"Xing","sequence":"additional","affiliation":[{"name":"Division of Hematology the First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000 PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Division of Hematology the First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000 PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijian","family":"Shen","sequence":"additional","affiliation":[{"name":"Division of Hematology the First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000 PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songfu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Division of Hematology the First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000 PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Yu","sequence":"additional","affiliation":[{"name":"Division of Hematology the First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000 PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Feng","sequence":"additional","affiliation":[{"name":"Division of Hematology the First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000 PR China"},{"name":"Division of Pediatric Hematology\u2010Oncology the First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000 PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,6,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2014.00054"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.11.1897"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1373518"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04584.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1547"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa062867"},{"key":"e_1_2_8_8_1","first-page":"3920","article-title":"Anti\u2010CD19 antibodies inhibit the function of the P\u2010gp pump in multidrug\u2010resistant B lymphoma cells","volume":"5","author":"Ghetie MA","year":"1999","journal-title":"Clin Cancer Res"},{"key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1182\/blood.V83.5.1329.1329","article-title":"Anti\u2010CD19 inhibits the growth of human B\u2010cell tumor lines in vitro and of Daudi cells in SCID mice by inducing cell cycle arrest","volume":"83","author":"Ghetie MA","year":"1994","journal-title":"Blood"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201489732"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-13-3274"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)62187-4"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1301361"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.6.1897"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.25690"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.21.9575"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.011114"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra052603"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-04-1616"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2008.07499.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2015.38"},{"key":"e_1_2_8_22_1","first-page":"957","article-title":"Incidence and clinical outcome of children with BCR\/ABL\u2010positive acute lymphoblastic leukemia (ALL). A prospective RT\u2010PCR study based on 673 patients enrolled in the German pediatric multicenter therapy trials ALL\u2010BFM\u201090 and CoALL\u201005\u201092","volume":"10","author":"Schlieben S","year":"1996","journal-title":"Leukemia"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(01)82002-9"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2007.07.002"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7531365"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellbi.2006.11.012"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbin.10994","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.10994","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T10:39:06Z","timestamp":1695033546000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.10994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,15]]},"references-count":25,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2018,9]]}},"alternative-id":["10.1002\/cbin.10994"],"URL":"https:\/\/doi.org\/10.1002\/cbin.10994","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,15]]},"assertion":[{"value":"2017-08-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}