{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T07:08:11Z","timestamp":1760425691454},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,2,6]],"date-time":"2019-02-06T00:00:00Z","timestamp":1549411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81371700","81501571"],"award-info":[{"award-number":["81371700","81501571"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label \/><jats:p>Our study was designed to investigate the effects of IL\u20107 during the differentiation process of adipose\u2010derived stem cells (ADSCs) toward lymphatic endothelial cells (LECs). IL\u20107 was added to the traditional induced medium, which was called the IL\u20107 (+) group, while the group that used traditional induced medium was called the IL\u20107 (\u2212) group. After 7 days of induction of ADSCs, a comprehensive analysis was conducted between these two groups. We examined the changes in Prox1, LYVE\u20101, Podoplanin and VEGFR\u20103 on the RNA and protein level and found that the expression of LEC markers in the IL\u20107 (+) group was higher than in the IL\u20107 (\u2212) group. The characteristics of differentiated cells were confirmed by flow cytometry and immunofluorescence. At the same time, we detected the MAPK\/ERK and PI3K\/AKT pathway involved in the differentiation process, and we found that the phosphorylation of AKT increased, however the expression of ERK was not significantly changed. In conclusion, our study found that IL\u20107 could improve the differentiation efficiency of ADSCs toward LECs through AKT signaling pathways.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.11093","type":"journal-article","created":{"date-parts":[[2019,1,29]],"date-time":"2019-01-29T03:45:18Z","timestamp":1548733518000},"page":"394-401","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["IL\u20107 enhances the differentiation of adipose\u2010derived stem cells toward lymphatic endothelial cells through AKT signaling"],"prefix":"10.1002","volume":"43","author":[{"given":"Yiyu","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200011 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bolun","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200011 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingcheng","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery the First Affiliated Hospital of Chongqing Medical University Chongqing 400016 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaohua","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200011 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weigang","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200011 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200011 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengli","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200011 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,6]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"721","article-title":"The effects of interleukin\u20107 on the lymphangiogenic properties of human endothelial cells","volume":"27","author":"Al\u2010Rawi MA","year":"2005","journal-title":"Int J Oncol"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.4832"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-5-55"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12425"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.793208"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2016.11.004"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/cell.2016.0038"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2006.09.125"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2003.12.109"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.02.127"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.2478\/s11658-013-0101-4"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1089\/lrb.2006.4403"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.02.051"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-01-478073"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/15476278.2016.1229726"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.5152\/turkjsurg.2017.3870"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.941468"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep11019"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2970"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2008.12.006"},{"key":"e_1_2_8_22_1","first-page":"134","article-title":"Current views on diagnostic approach and treatment of lymphedema","volume":"125","author":"Murdaca G","year":"2012","journal-title":"Am J Med Sci"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.112.000877"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.11.002"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/SAP.0000000000000084"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2015-0357"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.2217\/fon.11.121"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1089\/cell.2014.0043"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI71609"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.2217\/rme.15.24"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03511"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1089\/107632701300062859"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbin.11093","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.11093","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbin.11093","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.11093","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T11:38:46Z","timestamp":1694432326000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.11093"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,6]]},"references-count":31,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1002\/cbin.11093"],"URL":"https:\/\/doi.org\/10.1002\/cbin.11093","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,6]]},"assertion":[{"value":"2018-07-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}