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To date, much endeavor has been devoted to the fabrication of appropriate scaffolds with suitable biomechanical properties to support cell viability and growth in the microenvironment of a wound. The aim of this research was to assess the impact of adipose tissue\u2010derived mesenchymal stem cells (AD\u2010MSCs) and keratinocytes on gelatin\/chitosan\/\u03b2\u2010glycerol phosphate (GCGP) nanoscaffold in full\u2010thickness excisional skin wound healing of rats. For this purpose, AD\u2010MSCs and keratinocytes were isolated from rats and GCGP nanoscaffolds were electrospun. Through an in vivo study, the percentage of wound closure was assessed on days 7, 14, and 21 after wound induction. Samples were taken from the wound sites in order to evaluate the density of collagen fibers and vessels at 7 and 14 days. Moreover, sampling was done on days 7 and 14 from wound sites to assess the density of collagen fibers and vessels. The wound closure rate was significantly increased in the keratinocytes\u2010AD\u2010MSCs\u2010scaffold (KMS) group compared with other groups. The expressions of vascular endothelial growth factor, collagen type 1, and CD34 were also significantly higher in the KMS group compared with the other groups. These results suggest that the combination of AD\u2010MSCs and keratinocytes seeded onto GCGP nanoscaffold provides a promising treatment for wound healing.<\/jats:p>","DOI":"10.1002\/cbin.11119","type":"journal-article","created":{"date-parts":[[2019,2,21]],"date-time":"2019-02-21T14:51:02Z","timestamp":1550760662000},"page":"1365-1378","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Adipose tissue\u2010derived mesenchymal stem cells and keratinocytes co\u2010culture on gelatin\/chitosan\/\u03b2\u2010glycerol phosphate nanoscaffold in skin regeneration"],"prefix":"10.1002","volume":"43","author":[{"given":"Marzieh","family":"Lotfi","sequence":"first","affiliation":[{"name":"Department of Genetics, Faculty of Medicine Shahid Sadoughi University of Medical Sciences Yazd Iran"},{"name":"Department of Modern Sciences &amp; Technologies School of Medicine Mashhad University of Medical Sciences Mashhad 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