{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T02:21:23Z","timestamp":1790130083838,"version":"4.0.1"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T00:00:00Z","timestamp":1556582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2011\/03\/\/N\/NZ3\/05897"],"award-info":[{"award-number":["2011\/03\/\/N\/NZ3\/05897"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Transcription factor signal transducer and activator of transcription 3 (STAT3) is constitutively activated in many cancers, including melanomas. Active, phosphorylated STAT3 contributes to tumor growth and formation of the immunosuppressive tumor microenvironment. Recent evidence suggests an important role of STAT3 in self\u2010renewal of cancer stem\u2010like cells (CSCs). In the present study, we aimed to determine the expression and role of active STAT3 in melanoma CSCs. We found the increased levels of phosphorylated (Y705) STAT3 in CSC sphere cultures derived from three human and murine melanoma cells. Knockdown of STAT3 did not affect basal proliferation, but reduced sphere forming capacity of two human melanoma cell lines. Moreover, the level of active STAT3 was elevated in rhodamine 123 negative subpopulations of CSCs sorted from three melanoma cell lines. We found that focal adhesion kinase (FAK) and AKT signaling pathways, implicated in the regulation of cell migration and invasion, were up\u2010regulated in melanoma CSCs. Moreover, expression of SERPINA3<jats:italic>,<\/jats:italic> which regulates melanoma invasion, was increased in melanoma CSCs sphere cultures, which correlated with augmented cell invasion in Matrigel. Our findings show that STAT3 is activated and supports maintenance of melanoma CSCs. It suggests that STAT3 could serve as a potential target to impair tumor progression or recurrence.<\/jats:p>","DOI":"10.1002\/cbin.11134","type":"journal-article","created":{"date-parts":[[2019,4,8]],"date-time":"2019-04-08T16:22:16Z","timestamp":1554740536000},"page":"613-622","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Knockdown of STAT3 targets a subpopulation of invasive melanoma stem\u2010like cells"],"prefix":"10.1002","volume":"43","author":[{"given":"Dorota W","family":"Kulesza","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Neurobiology, Neurobiology Center The Nencki Institute of Experimental Biology Warsaw Poland"},{"name":"Postgraduate School of Molecular Medicine Warsaw Medical University Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Przanowski","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neurobiology, Neurobiology Center The Nencki Institute of Experimental Biology Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bozena","family":"Kaminska","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neurobiology, Neurobiology Center The Nencki Institute of Experimental Biology Warsaw Poland"},{"name":"Postgraduate School of Molecular Medicine Warsaw Medical University Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,30]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.23305"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2009.04911.x"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215617"},{"issue":"4","key":"e_1_2_7_1_5_1","first-page":"945","article-title":"Activated STAT signaling in human tumors provides novel molecular targets for therapeutic intervention","volume":"8","author":"Buettner R","year":"2002","journal-title":"Clin Cancer Res"},{"key":"e_1_2_7_1_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2012.0637"},{"key":"e_1_2_7_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80011-4"},{"key":"e_1_2_7_1_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-10-0504"},{"key":"e_1_2_7_1_9_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1606508"},{"issue":"8","key":"e_1_2_7_1_10_1","doi-asserted-by":"crossref","first-page":"3787","DOI":"10.4049\/jimmunol.158.8.3787","article-title":"Diversity of the cytotoxic melanoma\u2010specific immune response: Some CTL clones recognize autologous fresh tumor cells and not tumor cell lines","volume":"158","author":"Dufour E","year":"1997","journal-title":"J Immunol"},{"key":"e_1_2_7_1_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511605536"},{"issue":"12","key":"e_1_2_7_1_12_1","article-title":"STAT3, stem cells, cancer stem cells and p63","volume":"23","author":"Galoczova M","year":"2018","journal-title":"Cell Mol Biol Lett"},{"key":"e_1_2_7_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncponc0195"},{"key":"e_1_2_7_1_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-3195"},{"key":"e_1_2_7_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.25869"},{"key":"e_1_2_7_1_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M008503200"},{"key":"e_1_2_7_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2015.03.008"},{"key":"e_1_2_7_1_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.8.4406"},{"key":"e_1_2_7_1_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-005-1580-1"},{"key":"e_1_2_7_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2012.11.005"},{"key":"e_1_2_7_1_21_1","unstructured":"KuleszaDW RamjiK MaleszewskaM MieczkowskiJ DabrowskiM ChouaibS &KaminskaB(Unpublished). Search for novel STAT3\u2010dependent genes reveals SERPINA3 as a novel STAT3 target and its role invasion of human melanoma cells."},{"issue":"1","key":"e_1_2_7_1_22_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12943-016-0578-3","article-title":"Therapeutic implications of cellular and molecular biology of cancer stem cells in melanoma","volume":"16","author":"Kumar D","year":"2017","journal-title":"Mol Cancer"},{"key":"e_1_2_7_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780241"},{"key":"e_1_2_7_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.03.034"},{"issue":"12","key":"e_1_2_7_1_25_1","first-page":"3351","article-title":"Constitutive activation of signal transducers and activators of transcription 3 correlates with cyclin D1 overexpression and may provide a novel prognostic marker in head and neck squamous cell carcinoma","volume":"62","author":"Masuda M","year":"2002","journal-title":"Cancer Rese"},{"issue":"4","key":"e_1_2_7_1_26_1","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1097\/CMR.0b013e32836314e3","article-title":"The in\u2010vitro spheroid culture induces a more highly differentiated but tumorigenic population from melanoma cell lines","volume":"23","author":"Mo J","year":"2013","journal-title":"Melanoma Res"},{"key":"e_1_2_7_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2007.01.017"},{"key":"e_1_2_7_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2013.10.003"},{"key":"e_1_2_7_1_29_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.1143"},{"key":"e_1_2_7_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.06.610"},{"key":"e_1_2_7_1_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06489"},{"key":"e_1_2_7_1_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M700241200"},{"key":"e_1_2_7_1_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1333"},{"key":"e_1_2_7_1_34_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.111.188730"},{"key":"e_1_2_7_1_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2499"},{"key":"e_1_2_7_1_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm976"},{"key":"e_1_2_7_1_37_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.21952"},{"key":"e_1_2_7_1_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1108482108"},{"key":"e_1_2_7_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2011.01.002"},{"key":"e_1_2_7_1_40_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.9409"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.11134","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbin.11134","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.11134","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T14:10:23Z","timestamp":1694355023000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.11134"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,30]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,6]]}},"alternative-id":["10.1002\/cbin.11134"],"URL":"https:\/\/doi.org\/10.1002\/cbin.11134","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,30]]},"assertion":[{"value":"2018-08-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}