{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T20:16:11Z","timestamp":1767212171454},"reference-count":43,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,5,18]],"date-time":"2019-05-18T00:00:00Z","timestamp":1558137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The pathological mechanism underlying glaucoma has always been a complex aspect of this permanently blinding disease but proteomic studies have been helpful in elucidating it to a great extent in several studies. This study was designed to evaluate the expression and to get an idea about the function of two novel markers (ligatin and fibulin\u20107) identified in human aqueous humor (hAH) in relation to glaucomatous progression. A significant increase in the protein content of glaucomatous hAH compared to that of non\u2010glaucomatous controls (NG\u2010Ctrls) was observed. Ligatin, fibulin\u20107, and its proteolysis were revealed in hAH of primary open angle glaucoma (POAG), primary angle closure glaucoma (PACG) and NG\u2010Ctrls. Quantification confirmed no significant difference in expression of ligatin, whereas fibulin\u20107 was significantly (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) low in hAH of PACG in comparison to NG\u2010Ctrls and POAG. Importantly the immunohistochemical assay for both indicated their possible involvement in the maintenance of the appropriate structure of TM in vivo. Since oxidative stress is a major contributor to glaucomatous pathogenesis, <jats:italic>in vitro<\/jats:italic> analysis of nuclear and cytoplasmic fractions indicated intracellular changes in localization and expression of ligatin upon oxidative insult of human trabecular meshwork (TM) cells. While no such changes were found for fibulin\u20107 expression. This was\u00a0also corroborated with the immunocytochemical assay. Though a study with a small sample size, this\u00a0is the first report which confirms\u00a0the presence of ligatin and fibulin\u20107 in hAH, quantified their differential expression, and indicated the possibility of their involvement in the maintenance of the TM structure.<\/jats:p>","DOI":"10.1002\/cbin.11138","type":"journal-article","created":{"date-parts":[[2019,4,8]],"date-time":"2019-04-08T16:07:45Z","timestamp":1554739665000},"page":"820-834","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Possible role of differentially expressing novel protein markers (ligatin and fibulin\u20107) in human aqueous humor and trabecular meshwork tissue in glaucoma progression"],"prefix":"10.1002","volume":"43","author":[{"given":"Kaustuv","family":"Basu","sequence":"first","affiliation":[{"name":"Biochemistry and Environmental Toxicology Division Laboratory # 103, School of Environmental Sciences, Jawaharlal Nehru University New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Maurya","sequence":"additional","affiliation":[{"name":"Biochemistry and Environmental Toxicology Division Laboratory # 103, School of Environmental Sciences, Jawaharlal Nehru University New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jasbir","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Ocular Biochemistry Dr Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohit","family":"Saxena","sequence":"additional","affiliation":[{"name":"Glaucoma Research Facility &amp; Clinical Services Division Dr Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viney","family":"Gupta","sequence":"additional","affiliation":[{"name":"Glaucoma Research Facility &amp; Clinical Services Division Dr Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramanjit","family":"Sihota","sequence":"additional","affiliation":[{"name":"Glaucoma Research Facility &amp; Clinical Services Division Dr Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilora","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Biochemistry and Environmental Toxicology Division Laboratory # 103, School of Environmental Sciences, Jawaharlal Nehru University New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,5,18]]},"reference":[{"key":"e_1_2_10_1_2_1","first-page":"1891","article-title":"Alterations in the aqueous humor proteome in patients with aglaucoma shunt device","volume":"17","author":"Anshu A","year":"2011","journal-title":"Mol Vis"},{"key":"e_1_2_10_1_3_1","first-page":"1699","article-title":"Current primary open\u2010angle glaucoma treatments and future directions","volume":"6","author":"Beidoe G","year":"2012","journal-title":"Clin Ophthalmol"},{"key":"e_1_2_10_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.288"},{"key":"e_1_2_10_1_5_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.10-5531"},{"key":"e_1_2_10_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2007.10.033"},{"key":"e_1_2_10_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2012.09.008"},{"key":"e_1_2_10_1_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.10.7087"},{"key":"e_1_2_10_1_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.439158"},{"key":"e_1_2_10_1_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.119693"},{"key":"e_1_2_10_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0019-2791(71)90454-X"},{"key":"e_1_2_10_1_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000323584"},{"key":"e_1_2_10_1_13_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874364101004010052"},{"key":"e_1_2_10_1_14_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1995.01100100107039"},{"key":"e_1_2_10_1_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/02713683.2011.556299"},{"key":"e_1_2_10_1_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/IJG.0b013e3182946553"},{"key":"e_1_2_10_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2005.11.001"},{"issue":"2","key":"e_1_2_10_1_18_1","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1242\/jcs.93.2.227","article-title":"Molecular cloning of the cDNA for ligatin","volume":"93","author":"Jakoi ER","year":"1989","journal-title":"J Cell Sci"},{"issue":"1","key":"e_1_2_10_1_19_1","first-page":"3","article-title":"The juxtacanalicular region of ocular trabecular meshwork: a tissue with a unique extracellular matrix and specialized function","volume":"1","author":"Keller KE","year":"2013","journal-title":"J Ocul Biol"},{"key":"e_1_2_10_1_20_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2012-0048"},{"key":"e_1_2_10_1_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ijg.0000243480.67532.1b"},{"key":"e_1_2_10_1_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_10_1_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/APO.0000000000000180"},{"key":"e_1_2_10_1_24_1","doi-asserted-by":"publisher","DOI":"10.1159\/000489144"},{"key":"e_1_2_10_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90060-5"},{"key":"e_1_2_10_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.1982.240180407"},{"key":"e_1_2_10_1_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/9208489"},{"key":"e_1_2_10_1_28_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/3164734"},{"key":"e_1_2_10_1_29_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2005.081224"},{"issue":"4","key":"e_1_2_10_1_30_1","first-page":"451","article-title":"Molecular complexity of primary open angle glaucoma: current concepts","volume":"88","author":"Ray K","year":"2009","journal-title":"J Genet (Bangalore, INDIA)"},{"key":"e_1_2_10_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(83)34492-4"},{"key":"e_1_2_10_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)01127-8"},{"key":"e_1_2_10_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2006.10.008"},{"key":"e_1_2_10_1_34_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.123.4.458"},{"key":"e_1_2_10_1_35_1","doi-asserted-by":"crossref","first-page":"58.16","DOI":"10.4049\/jimmunol.190.Supp.58.16","article-title":"Fibulin\u20107, a member of the extracellular matrixfibulin family, regulates immune cell migration and function","volume":"190","author":"Sarangi P","year":"2013","journal-title":"J Immunol"},{"key":"e_1_2_10_1_36_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.003707"},{"issue":"80","key":"e_1_2_10_1_37_1","first-page":"17","article-title":"Genetic susceptibility to primary angle closure glaucoma (PACG)","volume":"15","author":"Shastry BS","year":"2013","journal-title":"Discov Med"},{"key":"e_1_2_10_1_38_1","doi-asserted-by":"publisher","DOI":"10.4103\/0301-4738.95868"},{"key":"e_1_2_10_1_39_1","unstructured":"Singh D (2014) Advances in plant biopesticides. Springer publication. ISBN: 978\u201081\u2010322\u20102005\u20103 (Print) 978\u201081\u2010322\u20102006\u20100 (Online)."},{"key":"e_1_2_10_1_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M705847200"},{"key":"e_1_2_10_1_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-009-8632-6"},{"key":"e_1_2_10_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2014.01.013"},{"key":"e_1_2_10_1_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2390"},{"key":"e_1_2_10_1_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2007.10.006"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.11138","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbin.11138","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.11138","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T19:41:40Z","timestamp":1694374900000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.11138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,18]]},"references-count":43,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1002\/cbin.11138"],"URL":"https:\/\/doi.org\/10.1002\/cbin.11138","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,18]]},"assertion":[{"value":"2018-11-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}