{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T03:55:37Z","timestamp":1788062137279,"version":"build-2784847793"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T00:00:00Z","timestamp":1556582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Periprosthetic osteolysis caused by wear particles is the main factor that affects\u00a0the long\u2010term efficacy of artificial joint replacement, and macrophages play\u00a0a vital role in the pathogenesis of periprosthetic osteolysis, while the potential mechanism underlying this\u00a0is\u00a0still unclear. To investigate the underlying role of miR\u2010377 in wear particle\u2010induced osteolysis (PIO), blood samples from patients undergoing arthroplasty were collected for analyzing the correlation between miR\u2010377 expression and the clinicopathological parameters of PIO. Peripheral blood macrophages were obtained to compare the miR\u2010377 and receptor activator of NF\u2010\u03baB ligand (RANKL) expressions. Bone marrow macrophages (BMMs) following titanium (Ti) particle treatment and\/or miR\u2010377 mimic transfection were\u00a0used. The expressions of RANKL, pro\u2010inflammatory cytokines interleukin 6 (IL\u20106) and tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) and the osteoclast\u2010related molecules tartrate\u2010resistant acid phosphatase (TRAP) and cathepsin K (CTSK) were determined using real\u2010time polymerase chain reaction or western blotting or enzyme\u2010linked immunosorbent assay or TRAP staining, when appropriate. The interaction between miR\u2010377 and RANKL was assessed by luciferase reporter assay. The in vivo role of miR\u2010377 in PIO was evaluated using a mouse calvarial osteolysis model. There were significant differences in downregulated miR\u2010377 expression between the different numbers of particles in the joint prostheses. The Ti particle treatment increased pro\u2010inflammatory cytokine levels, downregulated RANKL and increased osteoclast activity in BMMs, while miR\u2010377 overexpression led to the opposite effect. Taken together, miR\u2010377 downregulated the target gene RANKL, resulting in PIO inhibition. MiR\u2010377 relieved PIO by negatively regulating RANKL.<\/jats:p>","DOI":"10.1002\/cbin.11143","type":"journal-article","created":{"date-parts":[[2019,4,8]],"date-time":"2019-04-08T16:18:00Z","timestamp":1554740280000},"page":"658-668","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["MiR\u2010377 inhibits wear particle\u2010induced osteolysis via targeting RANKL"],"prefix":"10.1002","volume":"43","author":[{"given":"Wei","family":"Li","sequence":"first","affiliation":[{"name":"Department of Rehabilitation Medicine The Third Hospital of Hebei Medical University Shijiazhuang 050050 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Joint Surgery The Third Hospital of Hebei Medical University Shijiazhuang 050050 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