{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T10:00:37Z","timestamp":1771236037230,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,7,31]],"date-time":"2019-07-31T00:00:00Z","timestamp":1564531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To investigate the roles of tripartite motif containing 52 (TRIM52) in human hepatic fibrosis in vitro, human hepatic stellate cell line LX\u20102 cells were transfected with hepatitis B virus (HBV) replicon to establish HBV\u2010induced fibrosis in LX\u20102 cells, and then treated with small interfering RNA\u2010mediated knockdown of TRIM52 (siTRIM52). LX\u20102 cells without HBV replicon transfection were treated with lentiviruses\u2010mediated overexpression of TRIM52 and phosphatase magnesium dependent 1A (PPM1A). Fibrosis response of LX\u20102 cells were assessed by the production of hydroxyproline (Hyp) and collagen I\/III, as well as protein levels of \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA). PPM1A and phosphorylated (p)\u2010Smad2\/3 were measured to assess the mechanism. The correlation between TRIM52 and PPM1A was determined using co\u2010immunoprecipitation, and whether and how TRIM52 regulated the degradation of PPM1A were determined by ubiquitination assay. Our data confirmed HBV\u2010induced fibrogenesis of LX\u20102 cells, as evidenced by significant increase in Hyp and collagen I\/III and \u03b1\u2010SMA, which was associated with reduction of PPM1A and elevation of transforming growth factor\u2010\u03b2 (TGF\u2010\u03b2), p\u2010Smad2\/3, and p\u2010Smad3L. However, those changes induced by HBV were significantly attenuated with additional siTRIM52 treatment. Similar to HBV, overexpression of TRIM52 exerted promoted effect in the fibrosis of LX\u20102 cells. Interestingly, TRIM52 induced the fibrogenesis of LX\u20102 cells and the activation of TGF\u2010\u03b2\/Smad pathway were significantly reversed by PPM1A overexpression. Furthermore, our data confirmed TRIM52 as a deubiquitinase that influenced the accumulation of PPM1A protein, and subsequently regulated the fibrogenesis of LX\u20102 cells. TRIM52 was a fibrosis promoter in hepatic fibrosis in vitro, likely through PPM1A\u2010mediated TGF\u2010\u03b2\/Smad pathway.<\/jats:p>","DOI":"10.1002\/cbin.11206","type":"journal-article","created":{"date-parts":[[2019,7,22]],"date-time":"2019-07-22T12:40:43Z","timestamp":1563799243000},"page":"108-116","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Tripartite motif protein 52 (TRIM52) promoted fibrosis in LX\u20102 cells through PPM1A\u2010mediated Smad2\/3 pathway"],"prefix":"10.1002","volume":"44","author":[{"given":"Ju","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Infectious Disease First Affiliated Hospital of Kunming Medical University  Kunming 650032 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Lan","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease First Affiliated Hospital of Kunming Medical University  Kunming 650032 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease First Affiliated Hospital of Kunming Medical University  Kunming 650032 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine First Affiliated Hospital of Kunming Medical University  Kunming 650032 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"You","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease First Affiliated Hospital of Kunming Medical University  Kunming 650032 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan\u2010Mei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease First Affiliated Hospital of Kunming Medical University  Kunming 650032 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Ni","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine First Affiliated Hospital of Kunming Medical University  Kunming 650032 P. R. 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