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It is fast emerging as a scaffold\u00a0for multimeric signaling complexes with roles in tissue development and disease. The green fluorescent protein (GFP)\u2010tagged variants of SG2NA were ectopically expressed in NIH3T3 cells and their modulation by serum and GSK3\u03b2\u2010ERK signaling were monitored. The 87, 78, and 35\u2009kDa variants showed a biphasic modulation by serum till 24\u2009h but the 52\u2009kDa variant remained largely unresponsive. Inhibition of phosphatases by okadaic acid increased the levels of the endogenous 78\u2009kDa and the ectopically expressed GFP\u2010tagged 87 and 78\u2009kDa SG2NAs. Contrastingly, okadaic acid treatment reduced the level of GFP\u2010tagged 35\u2009kDa SG2NA, suggesting differential modes of their stability through phosphorylation\u2010dephosphorylation. The inhibition of GSK3\u03b2 by LiCl showed a gradual decrease in the levels of 78\u2009kDa. In the case of the other variants viz, GFP\u2010tagged 35, 52, and 87\u2009kDa, inhibition of GSK3\u03b2 caused an initial increase followed by a decrease with a subtle difference\u00a0in kinetics and intensities. Similar results were also seen upon inhibition of GSK3\u03b2 by small interfering RNA. All the variants showed an increase followed by a decrease upon inhibition\u00a0of extracellular\u2010signal\u2010regulated\u2010kinase (ERK). These variants are localized in the plasma membrane, endoplasmic reticulum, mitochondria, and the nucleus with different propensities and no discernable subcellular distribution was seen upon stimulation by serum and the inhibition of phosphatases, GSK3\u03b2, and ERK. Taken together, the variants of SG2NA are modulated by the kinase\u2010phosphatase network in a similar but characteristic manner.<\/jats:p>","DOI":"10.1002\/cbin.11264","type":"journal-article","created":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T04:04:31Z","timestamp":1574827471000},"page":"637-650","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Subcellular dynamics of variants of SG2NA in NIH3T3 fibroblasts"],"prefix":"10.1002","volume":"44","author":[{"given":"Pooja","family":"Chauhan","sequence":"first","affiliation":[{"name":"School of Life Sciences Jawaharlal Nehru University  New Mehrauli Road New Delhi 110067 India"},{"name":"Department of Microbiology and Molecular Genetics, Hadassah Medical School, Institute of Medical Research Israel\u2010Canada The Hebrew University of Jerusalem  POB 12272 Jerusalem 91120 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