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Mechanical stimuli are able to activate cellular responses by inducing the activation of several signaling pathways including cytoskeletal changes and inflammation. The actin cytoskeleton is a highly dynamic network and plays many important roles in intracellular events. Here, we aimed to investigate the involvement of a pivotal mediator of inflammatory responses, nuclear factor\u2010\u03baB (NF\u2010\u03baB), and actin polymerization in vibration\u2010induced upregulation of IL\u20106 and IL\u20108 expression in hPDL cells. hPDL cells were pretreated with the NF\u2010\u03baB inhibitor\u00a0BAY 11\u20107082 or cytochalasin D, respectively, before exposure to vibration. IL\u20106 and IL\u20108 messenger RNA (mRNA) and protein expression were quantified by quantitative polymerase chain reaction and enzyme\u2010linked immunosorbent assays, respectively. Subcellular localization of the NF\u2010\u03baB p65 subunit was visualized by immunofluorescent staining. We found an increase in NF\u2010\u03baB nuclear translocation in vibrated cells compared with control cells. Pretreatment with BAY 11\u20107082 significantly inhibited vibration\u2010induced IL\u20106 and IL\u20108 mRNA and protein expression in hPDL cells. Moreover, pretreatment with cytochalasin D inhibited NF\u2010\u03baB nuclear translocation and attenuated upregulation of IL\u20106 and IL\u20108 mRNA and protein in vibrated cells. Therefore, modulation of actin cytoskeletal polymerization in response to vibration may activate the NF\u2010\u03baB signaling pathway and subsequently upregulate IL\u20106 and IL\u20108 expression in hPDL cells.<\/jats:p>","DOI":"10.1002\/cbin.11267","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T10:35:38Z","timestamp":1574764538000},"page":"661-670","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Vibration activates the actin\/NF\u2010\u03baB axis and upregulates IL\u20106 and IL\u20108 expression in human periodontal ligament cells"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8519-1798","authenticated-orcid":false,"given":"Pussadee","family":"Phusuntornsakul","sequence":"first","affiliation":[{"name":"Trang Hospital  Muang Trang 92000 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