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In this study, we found that, no matter whether with vascular endothelial growth factor (VEGF) treatment or not, decreased expression of Dab2 could lead to cell apoptosis by preventing activation of VEGF\u2010VEGFR\u20102 signaling in GEnC, accompanied by reduced membrane VEGFR\u20102 expression. And silencing of PAR\u20103 gene expression caused increased apoptosis of GEnC by inhibiting activation of VEGF\u2010VEGFR\u20102 signaling and membrane VEGFR\u20102 expression. In our previous research, we found that the silencing of syndecan\u20101 gene expression inhibited VEGF\u2010VEGFR\u20102 signaling by modulating internalization of VEGFR\u20102. And our further research demonstrated that downregulation of syndecan\u20101 lead to no significant change in the expression of Dab2 and PAR\u20103 both at messenger RNA and protein levels in GEnC, while phosphorylation of Dab2 was significantly increased in GEnC transfected with Dab2 small interfering RNA (siRNA) compared with control siRNA. Atypical protein kinase C (aPKC) could induce phosphorylation of Dab2, thus negatively regulating VEGF\u2010VEGFR\u20102 signaling. And we found that decreased expression of syndecan\u20101 lead to activation of aPKC, and aPKC inhibitor treatment could block phosphorylation of Dab2 in GEnC. Besides, aPKC inhibitor treatment could activate VEGF\u2010VGEFR\u20102 signaling in GEnC transfected with syndecan\u20101 siRNA in a dose\u2010dependent manner. In conclusion, we speculated that phosphorylation of Dab2 is involved in preventing activation of VEGF\u2010VEGFR\u20102 signaling in GEnC transfected with syndecan\u20101 siRNA. This provides a new target for the therapy of GEnC injury and kidney disease.<\/jats:p>","DOI":"10.1002\/cbin.11288","type":"journal-article","created":{"date-parts":[[2019,12,23]],"date-time":"2019-12-23T11:18:30Z","timestamp":1577099910000},"page":"894-904","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Phosphorylation of Dab2 is involved in inhibited VEGF\u2010VEGFR\u20102 signaling induced by downregulation of syndecan\u20101 in glomerular endothelial cell"],"prefix":"10.1002","volume":"44","author":[{"given":"Zhou","family":"Jing","sequence":"first","affiliation":[{"name":"Department of Nephrology, Shanghai General Hospital Shanghai Jiao Tong University School of Medicine  100 HaiNing Road Shanghai 200080 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wu","family":"Jia\u2010jun","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Shanghai 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