{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:36:03Z","timestamp":1786977363220,"version":"build-2736575974"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T00:00:00Z","timestamp":1580083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004742","name":"National Academy of Sciences of Ukraine","doi-asserted-by":"publisher","award":["Grant 31\u201019"],"award-info":[{"award-number":["Grant 31\u201019"]}],"id":[{"id":"10.13039\/501100004742","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Many enzymes of methanol metabolism of methylotrophic yeasts are located in peroxisomes whereas some of them have the cytosolic localization. After shift of methanol\u2010grown cells of methylotrophic yeasts to glucose medium, a decrease in the activity of cytosolic (formaldehyde dehydrogenase, formate dehydrogenase, and fructose\u20101,6\u2010bisphosphatase [FBP]) along with peroxisomal enzymes of methanol metabolism is observed. Mechanisms of inactivation of cytosolic enzymes remain unknown. To study the mechanism of FBP inactivation, the changes in its specific activity of the wild type strain GS200, the strain with the deletion of the\n                    <jats:italic>GSS1<\/jats:italic>\n                    hexose sensor gene and strain defected in autophagy pathway SMD1163 of\n                    <jats:italic>Komagataella phaffii<\/jats:italic>\n                    with or without the addition of the MG132 (proteasome degradation inhibitor) were investigated after shift of methanol\u2010grown cells in glucose medium. Western blot analysis showed that inactivation of FBP in GS200 occurred due to protein degradation whereas inactivation in the strains SMD1163 and\n                    <jats:italic>gss1\u0394<\/jats:italic>\n                    was negligible in such conditions. The effect of the proteasome inhibitor MG132 on FBP inactivation was insignificant. To confirm FBP degradation pathway, the recombinant strains with GFP\u2010labeled Fbp1 of\n                    <jats:italic>K. phaffii<\/jats:italic>\n                    and red fluorescent protein\u2010labeled peroxisomes were constructed on the background of GS200 and SMD1163. The fluorescent microscopy analysis of the constructed strains was performed using the vacuolar membrane dye FM4\u201064. Microscopic data confirmed that Fbp1 degrades by autophagy pathway in\n                    <jats:italic>K. phaffii. K. phaffii<\/jats:italic>\n                    transformants, which express heterologous \u03b2\u2010galactosidase under\n                    <jats:italic>FLD<\/jats:italic>\n                    promoter, have been constructed.\n                  <\/jats:p>","DOI":"10.1002\/cbin.11304","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T02:36:33Z","timestamp":1578278193000},"page":"528-535","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Fructose\u20101,6\u2010bisphosphatase degradation in the methylotrophic yeast\n                    <i>Komagataella phaffii<\/i>\n                    occurs in autophagy pathway"],"prefix":"10.1002","volume":"45","author":[{"given":"Olena","family":"Dmytruk","sequence":"first","affiliation":[{"name":"Institute of Cell Biology National Academy of Science of Ukraine  Drahomanov Street 14\/16 79005 Lviv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Bulbotka","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology National Academy of Science of Ukraine  Drahomanov Street 14\/16 79005 Lviv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasya","family":"Zazulya","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology National Academy of Science of Ukraine  Drahomanov Street 14\/16 79005 Lviv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Semkiv","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology National Academy of Science of Ukraine  Drahomanov Street 14\/16 79005 Lviv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kostyantyn","family":"Dmytruk","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology National Academy of Science of Ukraine  Drahomanov Street 14\/16 79005 Lviv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andriy","family":"Sibirny","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology National Academy of Science of Ukraine  Drahomanov Street 14\/16 79005 Lviv Ukraine"},{"name":"Department of Biotechnology and Microbiology University of Rzeszow  Zelwerowicza 4 35\u2010601 Rzeszow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.14.9986"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M709922200"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.5.12.3376"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/3527602356"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.4161\/cib.27216"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1385\/0-89603-421-6:81","article-title":"Generation of protease\u2010deficient strains and their use in heterologous protein expression","volume":"103","author":"Gleeson MA","year":"1998","journal-title":"Methods Mol Biol"},{"issue":"1","key":"e_1_2_6_8_1","first-page":"95","article-title":"Regulation of gene expression in methylotrophic yeasts","volume":"59","author":"Grabek\u2010Lejko D","year":"2013","journal-title":"Postepy Biochem"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/143.4.1555"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404544200"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.04.026"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21284"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.12.018"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.363762"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.LAB.0000022226.23741.37"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1042\/CBI20100547"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2012.07.017"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0006297911120017"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/fow038"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.2144\/000112040"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.108.1.25"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.128.5.779"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.5.2527"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbin.11304","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.11304","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbin.11304","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.11304","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T05:51:15Z","timestamp":1693547475000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.11304"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,27]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,3]]}},"alternative-id":["10.1002\/cbin.11304"],"URL":"https:\/\/doi.org\/10.1002\/cbin.11304","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,27]]},"assertion":[{"value":"2019-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}