{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T05:50:41Z","timestamp":1771653041303,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T00:00:00Z","timestamp":1582588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Endometritis is a prevalent disease with inflammation of uterus endangering women reproductive health. MicroRNAs (miRNAs) play important roles in inflammatory disorders, including endometritis. However, the role and mechanism of miR\u2010643 in endometritis development remain unclear. This study aimed to investigate the effect of miR\u2010643 on lipopolysaccharide (LPS)\u2010induced inflammatory response and clarify the potential mechanism. LPS\u2010treated human endometrial epithelial cells (HEECs) were cultured to investigate the role of miR\u2010643 in vitro. The expression levels of miR\u2010643 and tumor necrosis factor receptor\u2010associated factor 6 (TRAF6) were measured via quantitative real\u2010time polymerase chain reaction and western blot, respectively. LPS\u2010induced inflammatory response was assessed by inflammatory cytokines secretion via enzyme\u2010linked immunosorbent assay. The activation of nuclear factor\u2010\u03baB (NF\u2010\u03baB) pathway was investigated by western blot. The interaction between miR\u2010643 and TRAF6 was validated by bioinformatics analysis, luciferase reporter assay, and RNA immunoprecipitation. The expression of miR\u2010643 was decreased and TRAF6 protein level was enhanced in LPS\u2010treated HEECs. The overexpression of miR\u2010643 suppressed LPS\u2010induced secretion of inflammatory cytokines (tumor necrosis factor\u2010\u03b1, interleukin\u20101\u03b2 [IL\u20101\u03b2], and IL\u20106) and activation of NF\u2010\u03baB pathway. The knockdown of TRAF6 inhibited LPS\u2010induced inflammatory response in HEECs. TRAF6 was validated as a target of miR\u2010643 and TRAF6 restoration reversed the effect of miR\u2010643 on inflammation response in LPS\u2010treated HEECs. Collectively, miR\u2010643 attenuated LPS\u2010induced inflammatory response by targeting TRAF6, indicating a novel avenue for the treatment of endometritis.<\/jats:p>","DOI":"10.1002\/cbin.11306","type":"journal-article","created":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T09:29:59Z","timestamp":1578907799000},"page":"1059-1067","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["MiR\u2010643 inhibits lipopolysaccharide\u2010induced endometritis progression by targeting TRAF6"],"prefix":"10.1002","volume":"44","author":[{"given":"Rui","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Gynecology of TCM Hainan Maternal and Children's Medical Center Haikou Hainan 571199 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gynecology of TCM Hainan Maternal and Children's Medical Center 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