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Due to their broad substrate specificity, they are widely used in many industrial processes and environmental bioremediations for removal of a large number of pollutants. During last decades, laccases attracted scientific interest also as highly promising enzymes to be used in bioanalytics. The aim of this study is to obtain a highly purified laccase from an efficient fungal producer and to demonstrate the applicability of this enzyme for analytics and bioremediation. To select the best microbial source of laccase, a screening of fungal strains was carried out and the fungus <jats:italic>Monilinia fructicola<\/jats:italic> was chosen as a producer of an extracellular enzyme. Optimal cultivation conditions for the highest yield of laccase were established; the enzyme was purified by a column chromatography and partially characterized. Molecular mass of the laccase subunit was determined to be near 35\u2009kDa; the optimal pH ranges for the highest activity and stability are 4.5\u20135.0 and 3.0\u20135.0, respectively; the optimal temperature for laccase activity is 30\u00b0C. Laccase preparation was successfully used as a biocatalyst in the amperometric biosensor for bisphenol A assay and in the bioreactor for bioremediation of some xenobiotics.<\/jats:p>","DOI":"10.1002\/cbin.11316","type":"journal-article","created":{"date-parts":[[2020,2,13]],"date-time":"2020-02-13T09:10:02Z","timestamp":1581585002000},"page":"536-548","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Extracellular laccase from <i>Monilinia fructicola<\/i>: isolation, primary characterization and application"],"prefix":"10.1002","volume":"45","author":[{"given":"Olga M.","family":"Demkiv","sequence":"first","affiliation":[{"name":"Institute of Cell Biology National Academy of Sciences of Ukraine 14\/16 Drahomanov Str. 79005 Lviv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina Z.","family":"Gayda","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology National Academy 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