{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T11:43:35Z","timestamp":1786707815370,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T00:00:00Z","timestamp":1590537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T00:00:00Z","timestamp":1590537600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Sonic hedgehog (Shh) signaling regulating epithelial\u2013mesenchymal transition (EMT) in cultured rat peritoneal mesothelial cells (PMCs) remains an under\u2010investigated topic. The current study aimed to elucidate the role of Shh signaling in the regulation of EMT in PMCs to attenuate peritoneal injury, with the view of enhancing the efficacy of peritoneal dialysis (PD). PMCs were initially extracted from male Wistar rats using pancreatic enzyme digestion. The expression of Shh and glioma\u2010associated oncogene homolog (Gli1) was quantitatively analyzed using the reverse\u2010transcription quantitative polymerase chain reaction (RT\u2010qPCR) and western blot analysis. Migration of PMCs was determined using Transwell assay. The expression of Shh, Gli1, and EMT markers including \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA), fibronectin, collagen I, snail1, and E\u2010cadherin was examined by RT\u2010qPCR, western blot analysis, and immunofluorescence respectively. High glucose induction was identified to promote cell migration and increase the expression of Shh and Gli1 in a dose\u2010 and time\u2010dependent manner in rat PMCs. Cyclopamine (CPN) was observed to block the Shh signaling induced by high glucose, accompanied by cell migration inhibition, decreased expression of \u03b1\u2010SMA, fibronectin, collagen I and snail1 as well as increased expression of E\u2010cadherin. Altogether, overexpression of Gli1 by transfected Gli1 plasmid promotes cell migration and upregulates \u03b1\u2010SMA, fibronectin, Snail1, and collagen I expression, while downregulating E\u2010cadherin expression. Shh\/Gli1 signaling is important in mediating EMT in rat PMCs, which provides a potential novel therapeutic approach for clinical investigation on renal failure treatment.<\/jats:p>","DOI":"10.1002\/cbin.11363","type":"journal-article","created":{"date-parts":[[2020,4,16]],"date-time":"2020-04-16T09:00:06Z","timestamp":1587027606000},"page":"1691-1700","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Regulation of epithelial\u2013mesenchymal transition via sonic hedgehog\/glioma\u2010associated oncogene homolog 1 signaling pathway in peritoneal mesothelial cells"],"prefix":"10.1002","volume":"44","author":[{"given":"Yanyan","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Nephrology The Fourth Affiliated Hospital of China Medical University  Shenyang Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nephrology The Fourth Affiliated Hospital of China Medical University  Shenyang Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Nephrology The Fourth Affiliated Hospital of China Medical University  Shenyang Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/jdb7020009"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2016.2546"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a028175"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2006030194"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevOncog.2017020473"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2016.4884"},{"issue":"2","key":"e_1_2_9_8_1","first-page":"662","article-title":"Glioma\u2010associated oncogene homolog 1 promotes epithelial\u2010mesenchymal transition in human renal tubular epithelial cell","volume":"8","author":"Ding H.","year":"2016","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-02063-x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.7236"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2017.12.007"},{"issue":"8","key":"e_1_2_9_12_1","first-page":"1602","article-title":"Critical role of glioma\u2010associated oncogene homolog 1 in maintaining invasive and mesenchymal\u2010like properties of melanoma cells","volume":"108","author":"Gunarta I. 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