{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T16:35:00Z","timestamp":1786379700713,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,6,22]],"date-time":"2020-06-22T00:00:00Z","timestamp":1592784000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous studies have shown that the tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) levels in serum and bone tissues formed in avascular necrosis of femoral head (ANFH) patients were higher than those\u00a0of normal individuals, indicating TNF\u2010\u03b1 might play a role in the pathogenesis of ANFH. However, the underlying mechanisms remain unclear. Hematoxylin and eosin staining was performed to show the pathological changes of ANFH bone tissues. TNF\u2010\u03b1 expression in normal and ANFH tissues was examined by quantitative real\u2010time polymerase chain reaction and western blot analyses. Osteoblast autophagy and apoptosis, as well as signaling pathways activation, were measured by their corresponding marker proteins. Osteoblast proliferation, autophagy, and apoptosis were evaluated using cell counting kit\u20108, transmission electron microscopy,\u00a0and flow cytometry. The structures of bone tissues of ANFH were obviously damaged. TNF\u2010\u03b1 expression was significantly upregulated in ANFH bone tissues compared to normal tissues. Autophagy and apoptosis were remarkably promoted, and p38 mitogen\u2010activated protein kinase (MAPK)\/nuclear factor\u2010\u03baB (NF\u2010\u03baB) signaling pathways were markedly activated in ANFH. Suppression of the p38 MAPK\/NF\u2010\u03baB pathway significantly attenuated the TNF\u2010\u03b1\u2010induced autophagy, however, enhanced the TNF\u2010\u03b1\u2010induced apoptosis in osteoblasts. Increased TNF\u2010\u03b1 in ANFH regulated osteoblast autophagy and apoptosis by p38 MAPK\/NF\u2010\u03baB signaling pathways, blocking the pathway by inhibitors exacerbated TNF\u2010\u03b1\u2010induced apoptosis through impairing\u00a0autophagy flux.<\/jats:p>","DOI":"10.1002\/cbin.11394","type":"journal-article","created":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T12:52:09Z","timestamp":1590065529000},"page":"1881-1889","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["TNF\u2010\u03b1 regulates the early development of avascular necrosis of the femoral head by mediating osteoblast autophagy and apoptosis via the p38 MAPK\/NF\u2010\u03baB signaling pathway"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0354-8341","authenticated-orcid":false,"given":"Li\u2010Wen","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Orthopedics, The Second Xiangya Hospital Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan\u2010Chun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, The Second Xiangya Hospital Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Zhan","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, The Second Xiangya Hospital Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Heng","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Radiology, The Second Xiangya Hospital Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong\u2010Yi","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Second Xiangya Hospital Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ding","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, The Second Xiangya Hospital Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.03.018"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2014.10.054"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-015-3026-7"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2013.11.028"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.JAA.0000450811.13707.78"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450117666160819155028"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-416618-9.00002-9"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M603783200"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep32092"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2014.06.013"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2012.82"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2014.09.001"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2013.08.036"},{"key":"e_1_2_9_15_1","first-page":"2065","article-title":"Autophagy inhibitor regulates apoptosis and proliferation of synovial fibroblasts through the inhibition of PI3K\/AKT pathway in collagen\u2010induced arthritis rat model","volume":"9","author":"Li S.","year":"2017","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3727\/096504016X14648701447779"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.00307"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2012-201671"},{"key":"e_1_2_9_19_1","first-page":"3215","article-title":"Combined effect of TNF\u2010alpha polymorphisms and hypoxia on steroid\u2010induced osteonecrosis of femoral head","volume":"8","author":"Liu Y.","year":"2015","journal-title":"International Journal of Clinical and Experimental Pathology"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-014-9773-0"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/svn-2016-000046"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-199503000-00018"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/13543776.2018.1502748"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5371"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10517-010-0873-8"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.1311.11057"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.15283\/ijsc17019"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.26374"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2013.11.019"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-017-3657-y"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-017-3639-0"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/6136205"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-018-2208-2"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-016-1199-0"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2014.02.016"},{"key":"e_1_2_9_36_1","first-page":"2143","article-title":"Effects of platelet\u2010rich plasma on angiogenesis and osteogenesis\u2010associated factors in rabbits with avascular necrosis of the femoral head","volume":"22","author":"Zhang X. L.","year":"2018","journal-title":"European Review for Medical and Pharmacological Sciences"},{"key":"e_1_2_9_37_1","first-page":"801","article-title":"microRNA206 contributes to the progression of steroidinduced avascular necrosis of the femoral head by inducing osteoblast apoptosis by suppressing programmed cell death 4","volume":"17","author":"Zhang Z.","year":"2018","journal-title":"Molecular Medicine Reports"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1136\/jim-2017-000426"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.11394","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbin.11394","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.11394","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T13:11:36Z","timestamp":1693919496000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.11394"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,22]]},"references-count":37,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1002\/cbin.11394"],"URL":"https:\/\/doi.org\/10.1002\/cbin.11394","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,22]]},"assertion":[{"value":"2019-09-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}