{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T15:48:27Z","timestamp":1785772107696,"version":"3.56.0"},"reference-count":38,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,7,6]],"date-time":"2020-07-06T00:00:00Z","timestamp":1593993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670746"],"award-info":[{"award-number":["81670746"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nucleotide\u2010binding domain, leucine\u2010rich repeat family with a caspase activation and recruitment domain 3 (NLRC3) participates in both immunity and cancer. The aim of this study was to determine the role of NLRC3 in human hepatocellular carcinoma (HCC) and the underlying mechanisms. We collected human liver tissues from nonalcoholic steatohepatitis (NASH), HCC, and adjacent normal tissues to characterize the pattern of NLRC3 expression by real\u2010time quantitative polymerase chain reaction and immunohistochemistry. Then, we used the HCC cell line, HuH\u20107, transfected with small interfering RNA to silence the NLRC3 expression. 5\u2010Ethynyl\u20102'\u2010deoxyuridine assay, scratch assay, and transwell invasion assay were used for assessing proliferation, migration, and invasion, respectively. Flow cytometry and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were conducted to assess cell apoptosis. The expression of NLRC3 was reduced in human HCC tissues, compared with normal liver and nonalcoholic steatohepatitis tissues. After knocking down of NLRC3, the proliferation, migration, and invasion were increased in HuH\u20107 cells. And flow cytometry and TUNEL assay showed that HuH\u20107 cell apoptosis was suppressed after NLRC3 knockdown. As for the underlying mechanisms, knockdown of NLRC3 in HuH\u20107 cells was associated with the activation of Janus kinase 2\/signal transducers and activators of transcription 3 (JAK2\/STAT3) pathway under interleukin\u20106 (IL\u20106) stimulation. NLRC3 expression was downregulated in human HCC tissues. NLRC3 silencing in HuH\u20107 cells can promote the proliferation, migration, and invasion of hepatocellular carcinoma cells. JAK2\/STAT3 pathway activation induced by IL\u20106 may be the underlying mechanism for HCC when NLRC3 expression is silenced. And the invasion of HuH\u20107 cells was partially suppressed by the STAT3 specific inhibitor (cryptotanshinone). Therefore, NLRC3 may play a significant role in HCC and might be a therapeutic target for the treatment of HCC.<\/jats:p>","DOI":"10.1002\/cbin.11414","type":"journal-article","created":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T14:40:31Z","timestamp":1593096031000},"page":"2053-2064","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["NLRC3 silencing accelerates the invasion of hepatocellular carcinoma cell via IL\u20106\/JAK2\/STAT3 pathway activation"],"prefix":"10.1002","volume":"44","author":[{"given":"Jian\u2010Hua","family":"Kang","sequence":"first","affiliation":[{"name":"Department of Endocrinology, Xinhua Hospital Shanghai Jiaotong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming\u2010Jie","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Xinhua Hospital Shanghai Jiaotong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei\u2010Pei","family":"Luan","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Shanghai Tenth People's Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De\u2010Ke","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Hepatology Unit, State Key Laboratory of Organ Failure Research, Guangdong Key Laboratory of Viral Hepatitis Research, Institutes of Liver Diseases Research of Guangdong Province, Nanfang Hospital Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan\u2010Wen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Xinhua Hospital Shanghai Jiaotong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Shanghai Tenth People's Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Shanghai Tenth People's Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Wei","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Shanghai Tenth People's Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Xinhua Hospital Shanghai Jiaotong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Hui","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Shanghai Tenth People's Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4421-4284","authenticated-orcid":false,"given":"Wei\u2010Xia","family":"Jian","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Xinhua Hospital Shanghai Jiaotong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-12629-4"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.28874"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.12.041"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21338"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-019-1193-0"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2018101397"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.5b00945"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2010.183"},{"key":"e_1_2_9_11_1","first-page":"2113","article-title":"TPX2 silencing exerts antitumor effects on hepatocellular carcinoma by regulating the PI3K\/AKT signaling pathway","volume":"44","author":"Huang D. 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