{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T18:45:52Z","timestamp":1785005152601,"version":"3.55.0"},"reference-count":50,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,8,16]],"date-time":"2020-08-16T00:00:00Z","timestamp":1597536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The pyroptosis is a causative agent of rheumatoid arthritis, a systemic autoimmune disease merged with degenerative articular cartilage. Nevertheless, the precise mechanism of extracellular acidosis on chondrocyte pyroptosis is largely unclear. Acid\u2010sensing ion channels (ASICs) belong to an extracellular H<jats:sup>+<\/jats:sup>\u2010activated cation channel family. Accumulating evidence has highlighted activation of ASICs induced by extracellular acidosis upregulate calpain and calcineurin expression in arthritis. In the present study, to investigate the expression and the role of acid\u2010sensing ion channel 1a (ASIC1a), calpain, calcineurin, and NLRP3 inflammasome proteins in regulating acid\u2010induced articular chondrocyte pyroptosis, primary rat articular chondrocytes were subjected to different pH, different time, and different treatments with or without ASIC1a, calpain\u20102, and calcineurin, respectively. Initially, the research results showed that extracellular acidosis\u2010induced the protein expression of ASIC1a in a pH\u2010 and time\u2010dependent manner, and the messenger RNA and protein expressions of calpain, calcineurin, NLRP3, apoptosis\u2010associated speck\u2010like protein, and caspase\u20101 were significantly increased in a time\u2010dependent manner. Furthermore, the inhibition of ASIC1a, calpain\u20102, or calcineurin, respectively, could decrease the cell death accompanied with the decreased interleukin\u20101\u03b2 level, and the decreased expression of ASIC1a, calpain\u20102, calcineurin, and NLRP3 inflammasome proteins. Taken together, these results indicated the activation of ASIC1a induced by extracellular acidosis could trigger pyroptosis of rat articular chondrocytes, the mechanism of which might partly be involved with the activation of calpain\u20102\/calcineurin pathway.<\/jats:p>","DOI":"10.1002\/cbin.11422","type":"journal-article","created":{"date-parts":[[2020,7,17]],"date-time":"2020-07-17T17:21:09Z","timestamp":1595006469000},"page":"2140-2152","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Acid\u2010sensing ion channel 1a mediates acid\u2010induced pyroptosis through calpain\u20102\/calcineurin pathway in rat articular chondrocytes"],"prefix":"10.1002","volume":"44","author":[{"given":"Sheng\u2010Qin","family":"Zu","sequence":"first","affiliation":[{"name":"Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Bin","family":"Feng","sequence":"additional","affiliation":[{"name":"Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan\u2010Jun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Shan","family":"Wu","sequence":"additional","affiliation":[{"name":"Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u2010Peng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge","family":"Li","sequence":"additional","affiliation":[{"name":"Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei\u2010Bei","family":"Dai","sequence":"additional","affiliation":[{"name":"Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Sen","family":"Wang","sequence":"additional","affiliation":[{"name":"Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Ya","family":"Xie","sequence":"additional","affiliation":[{"name":"Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Li","sequence":"additional","affiliation":[{"name":"Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Fang","family":"Ge","sequence":"additional","affiliation":[{"name":"Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei\u2010Hu","family":"Chen","sequence":"additional","affiliation":[{"name":"Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2012.068338"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.58"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-013-2128-8"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-015-0775-2"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)01936-3"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.12.031"},{"key":"e_1_2_11_8_1","first-page":"1778","article-title":"Inhibition of acidsensing ion channel 1a attenuates acidinduced activation of autophagy via a calcium signaling pathway in articular chondrocytes","volume":"43","author":"Gao W. 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