{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T05:32:37Z","timestamp":1766467957293,"version":"3.37.3"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T00:00:00Z","timestamp":1596499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["2015R1C1A2A01055015","2017R1D1A3B03033401","2018R1D1A1B07051064"],"award-info":[{"award-number":["2015R1C1A2A01055015","2017R1D1A3B03033401","2018R1D1A1B07051064"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nitric oxide (NO) and reactive oxygen species (ROS) have been shown to be linked with numerous diseases, including osteoarthritis (OA). Our study aimed to examine the effect of simvastatin on NO\u2010 or ROS\u2010induced cyclooxygenase\u20102 (COX\u20102) expression in OA. Simvastatin has attracted considerable attention since the discovery of its pharmacological effects on different pathogenic processes, including inflammation. Here, we report that simvastatin treatment blocked sodium nitroprusside (SNP)\u2010 and interleukin 1 beta (IL\u20101\u03b2)\u2010induced COX\u20102 production. In addition, simvastatin attenuated SNP\u2010induced NO production and IL\u20101\u03b2\u2010induced ROS generation. Treatment with simvastatin prevented SNP\u2010 and IL\u20101\u03b2\u2010induced nuclear factor kappa B (NF\u2010\u03baB) activity. Inhibiting NO production and ROS generation using <jats:italic>N<\/jats:italic>\u2010acetylcysteine (NAC) and NG\u2010monomethyl\u2010\n<jats:sc>l<\/jats:sc>\u2010arginine (\n<jats:sc>l<\/jats:sc>\u2010NMMA), respectively, accelerated the influence of simvastatin on NF\u2010\u03baB activity. In addition, NAC blocked SNP and simvastatin\u2010mediated COX\u20102 production and NF\u2010\u03baB activity but did not alter IL\u20101\u03b2 and simvastatin\u2010mediated COX\u20102 expression.\n<jats:sc>l<\/jats:sc>\u2010NMMA treatment also abolished IL\u20101\u03b2\u2010mediated COX\u20102 expression and NF\u2010\u03baB activation, whereas SNP and simvastatin\u2010mediated COX\u20102 expression were not altered compared with the levels in the SNP and simvastatin\u2010treated cells. Our findings suggested that simvastatin blocks COX\u20102 expression by inhibiting SNP\u2010induced NO production and IL\u20101\u03b2\u2010induced ROS generation by blocking the NF\u2010\u03baB pathway.<\/jats:p>","DOI":"10.1002\/cbin.11424","type":"journal-article","created":{"date-parts":[[2020,7,24]],"date-time":"2020-07-24T12:50:51Z","timestamp":1595595051000},"page":"2153-2162","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Simvastatin abolishes nitric oxide\u2010 and reactive oxygen species\u2010induced cyclooxygenase\u20102 expression by blocking the nuclear factor \u03baB pathway in rabbit articular chondrocytes"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3556-2199","authenticated-orcid":false,"given":"Seon\u2010Mi","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, College of Natural Sciences Kongju National University Gongju Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7991-3782","authenticated-orcid":false,"given":"Yohan","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, College of Natural Sciences Kongju National University Gongju Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6380-256X","authenticated-orcid":false,"given":"Song Ja","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, College of Natural Sciences Kongju National University Gongju Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,4]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_11_2_1","DOI":"10.7556\/jaoa.1999.99.11.S7"},{"doi-asserted-by":"publisher","key":"e_1_2_11_3_1","DOI":"10.1016\/S0008-6363(99)00115-7"},{"doi-asserted-by":"publisher","key":"e_1_2_11_4_1","DOI":"10.1172\/JCI11891"},{"doi-asserted-by":"publisher","key":"e_1_2_11_5_1","DOI":"10.1002\/art.10518"},{"doi-asserted-by":"publisher","key":"e_1_2_11_6_1","DOI":"10.1016\/S0006-2952(99)00296-8"},{"doi-asserted-by":"publisher","key":"e_1_2_11_7_1","DOI":"10.1016\/j.pmrj.2012.02.009"},{"doi-asserted-by":"publisher","key":"e_1_2_11_8_1","DOI":"10.1038\/aja.2012.51"},{"doi-asserted-by":"publisher","key":"e_1_2_11_9_1","DOI":"10.1152\/physrev.00018.2001"},{"doi-asserted-by":"publisher","key":"e_1_2_11_10_1","DOI":"10.1136\/ard.61.1.76"},{"doi-asserted-by":"publisher","key":"e_1_2_11_11_1","DOI":"10.1016\/S0140-6736(14)60802-3"},{"issue":"4","key":"e_1_2_11_12_1","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.4049\/jimmunol.146.4.1294","article-title":"Urinary nitrate excretion in relation to murine macrophage activation. Influence of dietary l\u2010arginine and oral NG\u2010monomethyl\u2010l\u2010arginine","volume":"146","author":"Granger D. L.","year":"1991","journal-title":"Journal of Immunology (Baltimore, Md.: 1950)"},{"doi-asserted-by":"publisher","key":"e_1_2_11_13_1","DOI":"10.1038\/nri1839"},{"doi-asserted-by":"publisher","key":"e_1_2_11_14_1","DOI":"10.1371\/journal.pone.0038917"},{"doi-asserted-by":"publisher","key":"e_1_2_11_15_1","DOI":"10.1016\/j.yexcr.2016.07.025"},{"doi-asserted-by":"publisher","key":"e_1_2_11_16_1","DOI":"10.5306\/wjco.v5.i4.677"},{"doi-asserted-by":"publisher","key":"e_1_2_11_17_1","DOI":"10.1093\/rheumatology\/kes363"},{"doi-asserted-by":"publisher","key":"e_1_2_11_18_1","DOI":"10.1016\/S1063-4584(03)00150-X"},{"doi-asserted-by":"publisher","key":"e_1_2_11_19_1","DOI":"10.1007\/s11010-011-1045-5"},{"doi-asserted-by":"publisher","key":"e_1_2_11_20_1","DOI":"10.1146\/annurev.pharmtox.45.120403.095748"},{"issue":"1","key":"e_1_2_11_21_1","first-page":"37","article-title":"Osteoarthritis pathogenesis\u2014A complex process that involves the entire joint","volume":"7","author":"Man G. S.","year":"2014","journal-title":"Journal of Medicine and Life"},{"doi-asserted-by":"publisher","key":"e_1_2_11_22_1","DOI":"10.2165\/11208240-000000000-00000"},{"doi-asserted-by":"publisher","key":"e_1_2_11_23_1","DOI":"10.1016\/j.niox.2008.12.005"},{"doi-asserted-by":"publisher","key":"e_1_2_11_24_1","DOI":"10.1016\/j.joca.2005.08.009"},{"doi-asserted-by":"publisher","key":"e_1_2_11_25_1","DOI":"10.1038\/sj.onc.1203239"},{"doi-asserted-by":"publisher","key":"e_1_2_11_26_1","DOI":"10.1016\/S1063-4584(96)80009-4"},{"doi-asserted-by":"publisher","key":"e_1_2_11_27_1","DOI":"10.3389\/fbioe.2018.00022"},{"doi-asserted-by":"publisher","key":"e_1_2_11_28_1","DOI":"10.1161\/ATVBAHA.110.207449"},{"doi-asserted-by":"publisher","key":"e_1_2_11_29_1","DOI":"10.1016\/j.joca.2006.04.008"},{"doi-asserted-by":"publisher","key":"e_1_2_11_30_1","DOI":"10.1016\/j.taap.2014.06.012"},{"doi-asserted-by":"publisher","key":"e_1_2_11_31_1","DOI":"10.1016\/j.joca.2016.11.011"},{"issue":"24","key":"e_1_2_11_32_1","first-page":"4728","article-title":"MiR\u201026b suppresses tumor cell proliferation, migration and invasion by directly targeting COX\u20102 in lung cancer","volume":"19","author":"Xia M.","year":"2015","journal-title":"European Review for Medical and Pharmacological Sciences"},{"doi-asserted-by":"publisher","key":"e_1_2_11_33_1","DOI":"10.1016\/j.yexcr.2013.08.026"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbin.11424","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.11424","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbin.11424","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.11424","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T05:02:26Z","timestamp":1693890146000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.11424"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,4]]},"references-count":32,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["10.1002\/cbin.11424"],"URL":"https:\/\/doi.org\/10.1002\/cbin.11424","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"type":"print","value":"1065-6995"},{"type":"electronic","value":"1095-8355"}],"subject":[],"published":{"date-parts":[[2020,8,4]]},"assertion":[{"value":"2020-01-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}