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In the present study, oxidized low\u2010density lipoprotein (ox\u2010LDL) activated the NLRP3 inflammasome in human RPE cell line ARPE\u201019. Our data showed that the expression of NLRP3, interleukin\u20101\u03b2 (IL\u20101\u03b2), and caspase\u20101 and the release of IL\u20101\u03b2 in ARPE\u201019 cells were substantially increased by ox\u2010LDL, whereas the addition of NLRP3 inhibitor INF39 dose\u2010dependently reversed the effect of ox\u2010LDL. Overexpression of tripartite motif\u2010containing protein 31 (TRIM31) also suppressed the effect of ox\u2010LDL in ARPE\u201019 cells. TRIM31 knockdown had similar effects with ox\u2010LDL but INF39 could block the effect of TRIM31 knockdown. Moreover, TRIM31 could interact with NLRP3 in ARPE\u201019 cells. Overexpression of TRIM31 increased NLRP3 ubiquitination. In conclusion, the results propose that TRIM31 could enhance NLRP3 ubiquitination, therefore inhibiting NLRP3 inflammasome and pyroptosis in human RPE cells.<\/jats:p>","DOI":"10.1002\/cbin.11429","type":"journal-article","created":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T11:53:00Z","timestamp":1595850780000},"page":"2213-2219","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["TRIM31 inhibits NLRP3 inflammasome and pyroptosis of retinal pigment epithelial cells through ubiquitination of NLRP3"],"prefix":"10.1002","volume":"44","author":[{"given":"Peirong","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Shanghai General Hospital (Shanghai First People's Hospital) Shanghai Jiao Tong University School of Medicine Shanghai China"},{"name":"Photomedicine Division Shanghai Engineering Center for Visual Science and Photomedicine Shanghai 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