{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T16:49:56Z","timestamp":1781714996446,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,9,22]],"date-time":"2020-09-22T00:00:00Z","timestamp":1600732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP17H06896"],"award-info":[{"award-number":["JP17H06896"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP190280A"],"award-info":[{"award-number":["JP190280A"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human dental pulp cells (HDPCs) play an important role in pulpitis. Semaphorin3A (Sema3A), which is an axon guidance molecule, is a member of the secretory semaphorin family. Recently, Sema3A has been reported to be an osteoprotective factor and to be involved in the immune response. However, the role of Sema3A in dental pulp inflammation remains unknown. The aim of this study was to reveal the existence of Sema3A in human dental pulp tissue and the effect of Sema3A which is released from tumor necrosis factor (TNF)\u2010\u03b1\u2010stimulated HDPCs on production of proinflammatory cytokines, such as interleukin (IL)\u20106 and CXC chemokine ligand 10 (CXCL10), from HDPCs stimulated with TNF\u2010\u03b1. Sema3A was detected in inflamed pulp as compared to normal pulp. HDPCs expressed Neuropilin\u20101(Nrp1) which is Sema3A receptor. TNF\u2010\u03b1 increased the levels of IL\u20106 and CXCL10 in HDPCs in time\u2010dependent manner. Sema3A inhibited production of these two cytokines from TNF\u2010\u03b1\u2010stimulated HDPCs. TNF\u2010\u03b1 induced soluble Sema3A production from HDPCs. Moreover, antibody\u2010based neutralization of Sema3A further promoted production of IL\u20106 and CXCL10 from TNF\u2010\u03b1\u2010stimulated HDPCs. Sema3A inhibited nuclear factor (NF)\u2010\u03baB P65 phosphorylation and inhibitor \u03baB\u03b1 degradation in TNF\u2010\u03b1\u2010stimulated HDPCs. These results indicated that Sema3A is induced in human dental pulp, and TNF\u2010\u03b1 acts on HDPCs to produce Sema3A, which partially inhibits the increase in IL\u20106 and CXCL10 production induced by TNF\u2010\u03b1, and that the inhibition leads to suppression of NF\u2010\u03baB activation. Therefore, it is suggested that Sema3A may regulate inflammation in dental pulp and be novel antiinflammatory target molecule for pulpitis.<\/jats:p>","DOI":"10.1002\/cbin.11466","type":"journal-article","created":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T00:48:35Z","timestamp":1600822115000},"page":"238-244","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Semaphorin3A released from human dental pulp cells inhibits the increase in interleukin\u20106 and CXC chemokine ligand 10 production induced by tumor necrosis factor\u2010\u03b1 through suppression of nuclear factor\u2010\u03baB activation"],"prefix":"10.1002","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0478-9363","authenticated-orcid":false,"given":"Satoru","family":"Shindo","sequence":"first","affiliation":[{"name":"Department of Biological Endodontics, Graduate School of Biomedical and Health Sciences Hiroshima University 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