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Previous studies have shown that stem cells inhibit cardiac hypertrophy; however, it is necessary to explore the mechanisms underlying this effect. This study aimed to investigate the possible mechanism underlying the inhibitory effect of BMSCs on cardiomyocyte hypertrophy. We induced cardiomyocyte hypertrophy in cultured rat cells through isoproterenol (ISO) treatment with or without BMSC coculture. A microarray was performed to analyze messenger RNA expression in response to ISO treatment and BMSC coculture. Pathway enrichment analysis showed that the expression of differential genes was closely related to the 5\u2032\u2010adenosine monophosphate\u2010activated protein kinase (AMPK) signaling pathway and that the expression of forkhead box O 1 (FoxO1) was significantly increased in the presence of BMSCs. Furthermore, we determined the expression levels of p\u2010AMPK\/AMPK and p\u2010FoxO1\/FoxO1 by western blot analysis. The expression of p\u2010AMPK\/AMPK was upregulated, whereas that of p\u2010FoxO1\/FoxO1 was downregulated upon coculturing with BMSCs. The AMPK\u2010specific antagonist Compound C inhibited the downregulation of p\u2010FoxO1\/FoxO1 induced by the BMSC coculture. Furthermore, treatment with the specific FoxO1 antagonist AS1842856 reduced the inhibitory effects of BMSCs on cardiomyocyte hypertrophy in vivo and in vitro. Our present study demonstrates the inhibition of cardiomyocyte hypertrophy by BMSCs, which occurs partly through the AMPK\u2013FoxO1 signaling pathway.<\/jats:p>","DOI":"10.1002\/cbin.11482","type":"journal-article","created":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T19:29:05Z","timestamp":1602617345000},"page":"188-197","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Bone marrow mesenchymal stem cells inhibit cardiac hypertrophy by enhancing FoxO1 transcription"],"prefix":"10.1002","volume":"45","author":[{"given":"Jiantao","family":"Qiu","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Surgery The Affiliated Hospital of Qingdao University Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaiteng","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The Affiliated Qingdao Municipal Hospital of Qingdao University Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunchang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of General Surgery Weifang Medical University Weifang Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weimin","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The Affiliated Qingdao Municipal Hospital of Qingdao University Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Mu","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The Affiliated Qingdao Municipal Hospital of Qingdao University Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangjun","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The Affiliated Qingdao Municipal Hospital of Qingdao University Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1493-8123","authenticated-orcid":false,"given":"Xueying","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The Affiliated Qingdao Municipal Hospital of Qingdao University Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2015-0032"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvaa023"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2010.73"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"C62","DOI":"10.1016\/j.jacc.2018.08.228","article-title":"GW29\u2010e1834 bone marrow mesenchymal stem cell\u2010derived exosomes attenuates pressure overload induced cardiac remodeling in vivo and in vitro","volume":"72","author":"Chen F.","year":"2018","journal-title":"Journal of the American College of Cardiology"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_201805_14973"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.13480"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00935.2011"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2010.229"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02795-4"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1548-7"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051632"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.01.070"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755-5922.12248"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.302364"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.304707"},{"key":"e_1_2_10_17_1","volume-title":"Cardiac electrical and structural remodeling","author":"Liaquat M. 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