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Inhibition of intracellular transport causes lipid droplet retention and abnormal protein distribution. The intracellular transport of synthesized lipids and proteins from the endoplasmic reticulum (ER) to the Golgi apparatus is performed by coat complex II (COPII) vesicle transport, but how CCl<jats:sub>4<\/jats:sub> inhibits COPII vesicle transport has not been elucidated. COPII vesicle formation on the ER membrane is initiated by the recruitment of Sar1 protein from the cytoplasm to the ER membrane, followed by that of the COPII coat constituent proteins (Sec23, Sec24, Sec13, and Sec31). In this study, we evaluated the effect of CCl<jats:sub>4<\/jats:sub> on COPII vesicle formation using the RLC\u201016 rat hepatocyte cell line. Our results showed that CCl<jats:sub>4<\/jats:sub> suppressed ER\u2010Golgi transport in RLC\u201016 cells. Using a reconstituted system of rat liver tissue\u2010derived cytoplasm and RLC\u201016 cell\u2010derived ER membranes, CCl<jats:sub>4<\/jats:sub> treatment inhibited the recruitment of Sar1 and Sec13 from the cytosolic fraction to ER membranes. CCl<jats:sub>4<\/jats:sub>\u2010induced changes in the ER membrane accordingly inhibited the accumulation of COPII vesicle\u2010coated constituent proteins on the ER membrane, as well as the formation of COPII vesicles, which suppressed lipid and protein transport between the ER and Golgi apparatus. Our data suggest that CCl<jats:sub>4<\/jats:sub> inhibits ER\u2010Golgi intracellular transport by inhibiting COPII vesicle formation on the ER membrane in hepatocytes.<\/jats:p>","DOI":"10.1002\/cbin.11510","type":"journal-article","created":{"date-parts":[[2020,11,28]],"date-time":"2020-11-28T11:36:35Z","timestamp":1606563395000},"page":"633-641","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Carbon tetrachloride suppresses ER\u2010Golgi transport by inhibiting COPII vesicle formation on the ER membrane in the RLC\u201016 hepatocyte cell line"],"prefix":"10.1002","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9451-4736","authenticated-orcid":false,"given":"Hiroshi","family":"Nakagawa","sequence":"first","affiliation":[{"name":"Laboratory of Toxicology, Graduate School of Veterinary Sciences Osaka Prefecture University Izumisano Osaka 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