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However, little\u00a0is known about the potential functions of circRNAs in essential thrombocythemia (ET) development. The circRNA profile\u00a0alterations in the bone marrow of ET patients were mainly investigated in this study. The sizes of exosomes derived from human bone marrow tissues were validated by the nanoparticle tracking analysis (NTA) method. CD63 and TSG101 expressions in exosomes were analyzed by western blot analysis. The profiles and differential expression of circRNAs in bone\u2010derived exosomes were characterized by high\u2010throughput sequencing. Herein, circular structures and expression of circRNAs were verified by Sanger sequencing and real\u2010time polymerase chain reaction, respectively. The circRNA\u2010miRNA\u2010mRNA networks were predicted using the Cytoscape software. And we detected the effect of circ_0014614 on the transformation of K562 cells into megakaryocytes. Exosomes derived from the bone marrow of ET patients and healthy volunteers showed a diameter between 70 and 140\u2009nm and expressed high CD63 and TSG101. Meanwhile, the circRNA profiles were significantly altered in bone\u00a0marrow\u2013derived exosomes from ET patients, among which circDAP3, circASXL1, and circRUNX1 were significantly downregulated in ET patients, thus conferring a new insight into the role of circRNAs in the pathogenesis of ET. Besides this, circRNA\u2010encoding genes and miRNA\u2010mRNA networks targeted by this three circRNA were involved in various biological processes and signaling pathways. And circ_0014614 could inhibit K562 cells' differentiation into megakaryocytes. The predictions of the potential function of these three differentially expressed circRNAs along with their interaction with specific miRNAs\u00a0could provide a basis for circRNA\u2010based ET diagnosis and treatment.<\/jats:p>","DOI":"10.1002\/cbin.11534","type":"journal-article","created":{"date-parts":[[2020,12,16]],"date-time":"2020-12-16T07:34:12Z","timestamp":1608104052000},"page":"869-881","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Differential expression of circular RNAs in bone marrow\u2010derived exosomes from essential thrombocythemia patients"],"prefix":"10.1002","volume":"45","author":[{"given":"Qiang","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Hematology,\u00a0Nanfang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guopan","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Hematology,\u00a0Nanfang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"He","sequence":"additional","affiliation":[{"name":"Department of Hematology,\u00a0Nanfang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongxin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Hematology,\u00a0Nanfang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hematology,\u00a0Nanfang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Hematology,\u00a0Nanfang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9477-9286","authenticated-orcid":false,"given":"Dan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Hematology,\u00a0Nanfang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,31]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12926"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-018-0838-8"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0b013e3283497f54"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/bcj.2016.81"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-07-661983"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.092411"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428194.2017.1312371"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3074"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/5135781"},{"issue":"004","key":"e_1_2_12_11_1","first-page":"616","article-title":"The research progress of circular RNA and hematopoiesis and the relationship of hematological malignancies","volume":"026","author":"Fang C.","year":"2018","journal-title":"Journal\u00a0of Modern Oncology"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.16881"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0409-z"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29270"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2014.11.009"},{"issue":"005","key":"e_1_2_12_16_1","first-page":"46","article-title":"Effects of Bacopa monnieri saponins on the differentiation of K562 cells into megakaryocytes and related gene regulatory mechanisms","volume":"035","author":"Jiang X. 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