{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:36:45Z","timestamp":1740105405836,"version":"3.37.3"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,1,7]],"date-time":"2021-01-07T00:00:00Z","timestamp":1609977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2014\/50938\u20108"],"award-info":[{"award-number":["2014\/50938\u20108"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Prostate cancer is a life\u2010threatening condition worldwide. As the tumor progresses, smooth muscle cells (SMCs) become atrophic\/dedifferentiated, within a series of stromal changes named stromal reaction. Here, we tested whether a laminin 111\u2010rich extracellular matrix (Lr\u2010ECM) could affect SMCs phenotype and differentiation status. Using time\u2010lapse microscopy, image analyses, quantitative real\u2010time reverse transcription polymerase chain reaction, immunohistochemistry and immunoblotting, and transmission electron microscopy, we showed that SMCs acquires a migratory behavior with a decreased expression of differentiation markers and relocation of focal adhesion kinase. SMCs set homotypic cell junctions and were active in autophagy\/phagocytosis. Analysis of the migratory behavior showed that SMCs polarized and migrated toward each other, recognizing long\u2010distance signals such as matrix tensioning. However, half of the cell population were immotile, irrespective of the nearest neighbor distance, suggesting they do not engage in productive interactions, possibly as a result of back\u2010to\u2010back positioning. In conclusion, the Lr\u2010ECM, mimics the effects of the proliferating and infiltrating tumor epithelium, causing SMCs phenotypical change similar to that observed in the stromal reaction, in addition to a hitherto undescribed, stereotyped pattern of cell motility resulting from cell polarization.<\/jats:p>","DOI":"10.1002\/cbin.11535","type":"journal-article","created":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T15:30:01Z","timestamp":1609342201000},"page":"882-889","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Polarization, migration, and homotypical interactions among prostatic smooth muscle cells in a laminin 111\u2010rich extracellular matrix"],"prefix":"10.1002","volume":"45","author":[{"given":"Daniel A.","family":"Os\u00f3rio","sequence":"first","affiliation":[{"name":"Department of Structural and Functional Biology, Institute of Biology State University of Campinas (UNICAMP) Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvio R.","family":"Consonni","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Tissue Biology, Institute of Biology State University of Campinas (UNICAMP) Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline M.","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology, Institute of Biology State University of Campinas (UNICAMP) Campinas S\u00e3o Paulo Brazil"},{"name":"National Institute of Photonics Applied to Cell Biology State University of Campinas (UNICAMP) Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3080-9447","authenticated-orcid":false,"given":"Hernandes F.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology, Institute of Biology State University of Campinas (UNICAMP) Campinas S\u00e3o Paulo Brazil"},{"name":"National Institute of Photonics Applied to Cell Biology State University of Campinas (UNICAMP) Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.22684"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.107.002873"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2004.tb02758.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.28544"},{"key":"e_1_2_10_6_1","first-page":"437","article-title":"Analytical molecular pathology of epithelial\u2013stromal interactions in the normal and neoplastic prostate","volume":"20","author":"Bonkhoff H.","year":"1998","journal-title":"Analytical and Quantitative Cytology and Histology"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2009.01004.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091720307"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.96.6.1662"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2017.02.019"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(02)00395-4"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1139\/bcb-79-4-525"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.05.050"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000147686"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100818-125157"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.137.3.8603596"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2008.03.016"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11491"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400797"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/284067a0"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_10_22_1","unstructured":"Rodriguez D. A. O. Consonni S. R. Felisbino S. L. Santos A. M. &Carvalho H. F.(2020). Type I collagen triggers fast adhesion and favors prostatic smooth muscle cell hypertrophy in response to androgens under review."},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0097080"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1019"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2014.02.021"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.20728"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)65620-0"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60437-6"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.49.3.614"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.11535","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbin.11535","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.11535","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T16:48:05Z","timestamp":1693327685000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.11535"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,7]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,4]]}},"alternative-id":["10.1002\/cbin.11535"],"URL":"https:\/\/doi.org\/10.1002\/cbin.11535","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"type":"print","value":"1065-6995"},{"type":"electronic","value":"1095-8355"}],"subject":[],"published":{"date-parts":[[2021,1,7]]},"assertion":[{"value":"2020-09-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}