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However, the effects and mechanisms of curcumin on articular chondrocyte injury remain to be elucidated. The aim of the present study is to investigate the chondroprotective mechanisms of curcumin on interleukin\u20101\u03b2 (IL\u20101\u03b2)\u2010induced chondrocyte apoptosis in vitro. The results revealed that IL\u20101\u03b2 decreased cell viability and induced apoptosis in primary articular chondrocytes. Curcumin pretreatment reduced IL\u20101\u03b2\u2010induced articular chondrocyte apoptosis. In addition, treatment with curcumin increased autophagy in articular chondrocytes and protected against IL\u20101\u03b2\u2010induced apoptosis. The curcumin\u2010mediated protection against IL\u20101\u03b2 induced apoptosis was abolished when cells were treated with the autophagy inhibitor 3\u2010methyladenine or transfected with Beclin\u20101 small interfering RNA. Furthermore, IL\u20101\u03b2 stimulation significantly increased the phosphorylation levels of nuclear factor (NF)\u2010\u03baB p65 and glycogen synthase kinase\u20103\u03b2, and decreased the phosphorylation levels of \u03b2\u2010catenin in articular chondrocytes, and these alterations to the phosphorylation levels were partly reversed by treatment with curcumin. Dual\u2010luciferase and electrophoretic mobility shift assays demonstrated that IL\u20101\u03b2 increased NF\u2010\u03baB p65 promoter activity in chondrocytes, and this was also reversed by curcumin. Pretreatment with the NF\u2010\u03baB inhibitor pyrrolidine dithiocarbamate enhanced the protective effects of curcumin on chondrocyte apoptosis, but Wnt\/\u03b2\u2010catenin inhibitor, XAV\u2010939, did not exhibit this effect. Molecular docking and dynamic simulation studies results showed that curcumin could bound to RelA (p65) protein. These results indicate that curcumin may suppress IL\u20101\u03b2\u2010induced chondrocyte apoptosis through activating autophagy and restraining NF\u2010\u03baB signaling pathway.<\/jats:p>","DOI":"10.1002\/cbin.11541","type":"journal-article","created":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T15:29:49Z","timestamp":1609342189000},"page":"976-988","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Curcumin ameliorates IL\u20101\u03b2\u2010induced apoptosis by activating autophagy and inhibiting the NF\u2010\u03baB signaling pathway in rat primary articular chondrocytes"],"prefix":"10.1002","volume":"45","author":[{"given":"Tianyi","family":"Chen","sequence":"first","affiliation":[{"name":"School of Life and Medical Sciences University of Hertfordshire Hatfield 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