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This study aimed to investigate the effect of human umbilical cord MSCs (hUC\u2010MSCs) transfected with tissue inhibitors of matrix metalloproteinase (TIMP)\u20101 on the regeneration of \u03b2\u2010cell islets in vitro and in vivo. hUC\u2010MSCs were isolated, cultured, and transfected with lentiviruses for the overexpression of hTIMP\u20101. An in vitro coculture system of hUC\u2010MSCs and streptozotocin\u2010induced islets was established to examine the morphology, apoptosis, and insulin secretion of the cocultured islets. Diabetic mouse models were injected with lenti\u2010TIMP\u20101\u2010enhanced green fluorescent protein (EGFP)\u2010hUC\u2010MSCs to test the effect of hTIMP\u20101 on insulin levels and glucose tolerance in vivo. The expression of insulin and glucagon was evaluated by immunofluorescence staining. The results showed that coculture with hUC\u2010MSCs or Lenti\u2010TIMP\u20101\u2010EGFP\u2010hUC\u2010MSCs improved islet viability rates. Lenti\u2010TIMP\u20101\u2010EGFP\u2010hUC\u2010MSC coculture increased the insulin and C\u2010peptide secretion function of the cultured islets and increased the secretion of tumor necrosis factor\u2010\u03b21, interleukin\u20106, IL\u201010, and hTIMP\u20101. hUC\u2010MSCs, especially those transfected with Lenti\u2010hTIMP\u20101\u2010EGFP, showed a strong protective effect in diabetic mice by alleviating weight loss and improving glucose and insulin metabolism. In addition, transplantation rescued islet histology and function in vivo. The overexpression of TIMP\u20101 by hUC\u2010MSCs seems to exert beneficial effects on pancreatic islet cells. In conclusion, this study may provide a new perspective on the development of hUC\u2010MSC\u2010based cell transplantation therapy for T1DM.<\/jats:p>","DOI":"10.1002\/cbin.11548","type":"journal-article","created":{"date-parts":[[2021,1,7]],"date-time":"2021-01-07T19:22:09Z","timestamp":1610047329000},"page":"1038-1049","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Effect of hTIMP\u20101 overexpression in human umbilical cord mesenchymal stem cells on the repair of pancreatic islets in type\u20101 diabetic mice"],"prefix":"10.1002","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5992-3323","authenticated-orcid":false,"given":"Yu","family":"Bao","sequence":"first","affiliation":[{"name":"Department of Nephrology, National Clinical Research Center for Child Health, The Children's Hospital Zhejiang University School of Medicine  Hangzhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Clinic of Division of Child Health Care, National Clinical Research Center for Child Health, The Children's Hospital Zhejiang University School of Medicine  Hangzhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huihui","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pediatric and Adolescent Gynaecology, National Clinical Research Center for Child Health, The Children's Hospital Zhejiang University School of Medicine  Hangzhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.TP.0000128334.93343.B3"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20171251"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.4274\/tjh.galenos.2019.2019.0439","article-title":"Comparison of different culture conditions for mesenchymal stem cells from human umbilical cord Wharton's Jelly for stem cell therapy","volume":"37","author":"Bao Y.","year":"2020","journal-title":"Turkish journal of haematology: official journal of Turkish Society of Haematology"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevBiomedEng.2018027377"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68341-4"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/19382014.2017.1286434"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368912X647162"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0206449"},{"key":"e_1_2_9_10_1","first-page":"4479","article-title":"Stem cell transplantation for the treatment of patients with type 1 diabetes mellitus: A meta\u2010analysis","volume":"16","author":"Gan J.","year":"2018","journal-title":"Experimental and Therapeutic Medicine"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.50.5.1047"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11892-019-1213-7"},{"key":"e_1_2_9_13_1","first-page":"55","article-title":"Immune\u2010modulatory changes after transplantation therapy of insulin producing cells derived from Wharton's jelly human umbilical cord\u2010mesenchymal stem cells in diabetes induced rats","volume":"26","author":"Hassanin O. 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