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LIM domain kinase 1 (Limk1) is reported as a pro\u2010fibrotic mediator in patients with permanent atrial fibrillation and it has also been suggested to aggravate cardiac dysfunction in rats with chronic heart failure. The present study observed that Limk1 was significantly upregulated in the in vitro model of CH induced by angiotensin II (Ang\u2010II). Interestingly, silencing Limk1 led to inhibition of\u00a0the hypertrophic phenotypes in Ang\u2010II\u2010treated cardiomyocytes. Next, through a series of mechanistic assays including RIP assay, RNA pull\u2010down assay, and luciferase reporter assay, miR\u201093\u20105p was verified to target Limk1. Furthermore, circ\u2010Zfp644 was validated as the sponge of miR\u201093\u20105p. Circ\u2010Zfp644 functioned as a ceRNA to upregulate Limk1 expression via sequestering miR\u201093\u20105p in Ang\u2010II\u2010treated cardiomyocytes. Finally, a range of rescue assays revealed that circ\u2010Zfp644 stimulated hypertrophic effects in Ang\u2010II\u2010treated cardiomyocytes via upregulating Limk1 while miR\u201093\u20105p exerted the opposite effects via its inhibition on Limk1. On the whole, the present study revealed that circ\u2010Zfp644 aggravated CH through modulating the miR\u201093\u20105p\/Limk1 axis. The findings observed on the in vitro model of CH provided new potential for developing CH treatment.<\/jats:p>","DOI":"10.1002\/cbin.11569","type":"journal-article","created":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T05:04:59Z","timestamp":1612933499000},"page":"1260-1268","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Circ\u2010Zfp644 acts as a pro\u2010hypertrophic mediator in an Ang\u2010II induced in vitro myocardial hypertrophy model"],"prefix":"10.1002","volume":"45","author":[{"given":"Yongquan","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Cardiology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daifu","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Cardiology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 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