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However, there have been no studies, to our knowledge, regarding 4\u2010mu treatment in pulp inflammation. Therefore, the purpose of this study was to investigate the effects of 4\u2010mu on biological behaviors in human dental pulp stem cells (hDPSCs) exposed to lipopolysaccharide (LPS) in vitro. hDPSCs were exposed to LPS to construct the inflammation model in vitro. Immunocytochemistry, quantitative polymerase chain reaction, western blotting, Cell Counting Kit\u20108, scratch\/Transwell assay, and alizarin red staining\/alkaline phosphatase staining were selected to explore the effect of 4\u2010mu on the expression of inflammatory factors, cell proliferation, cell migration, and the odontogenic differentiation ability of hDPSCs. LPS stimulated hDPSCs to highly express the related inflammatory factors and CD44 (the major HA receptor), which were all inhibited by 0.1\u2009mM of 4\u2010mu. In addition, the cell proliferation ability of hDPSCs was suppressed by 4\u2010mu, while cell migration and odontogenic differentiation abilities were significantly improved under inflammation. In conclusion, 4\u2010mu suppressed inflammatory cytokines in inflamed hDPSCs and had a positive effect on the migration and odontogenic differentiation of hDPSCs.<\/jats:p>","DOI":"10.1002\/cbin.11579","type":"journal-article","created":{"date-parts":[[2021,3,6]],"date-time":"2021-03-06T12:07:53Z","timestamp":1615032473000},"page":"1415-1422","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["4\u2010Methylumbelliferone promotes the migration and odontogenetic differentiation of human dental pulp stem cells exposed to lipopolysaccharide in vitro"],"prefix":"10.1002","volume":"45","author":[{"given":"Weiting","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry, School &amp; Hospital of Stomatology Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration  Shanghai 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