{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T15:34:06Z","timestamp":1793028846832,"version":"4.5.0"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T00:00:00Z","timestamp":1620000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81503609"],"award-info":[{"award-number":["81503609"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Endometriosis is an inflammation\u2010dependent disease that shares similarities with malignant tumors including attachment and infiltration. Tripartite motif\u2010containing 24 (TRIM24) has been illustrated in inflammatory responses and gynecological tumors, and Nod\u2010like receptor protein 3 (NLRP3) inflammasome has been implicated in endometriosis. However, the involvement of TRIM24 and the role of NLRP3\/caspase\u20101\/interleukin\u20101\u03b2 (IL\u20101\u03b2)\u2010mediated pyroptosis in endometriosis remain obscure. In this study, we originally detected the decreased expression of TRIM24 in the ectopic endometrium of endometriosis\u00a0compared with the normal endometrium. Then we measured the promoted protein expression of pyroptotic biomarkers (NLRP3, procaspase\u20101, caspase\u20101, pro\u2010IL\u20101\u03b2, and IL\u20101\u03b2) using Western blot analysis\u00a0and the stimulated secretion of IL\u20101\u03b2 and IL\u201018 by enzyme\u2010linked immunosorbent assay\u00a0in ectopic human endometrial stromal cells (hESC) compared with normal hESC. TRIM24\u2010small\u2010interfering RNA (siTRIM24) was used to silence TRIM24, whereas TRIM24\u2010pcDNA3.1 was used for overexpressing TRIM24. The migration of hESC was determined by a Transwell migration assay. Coimmunoprecipitation and ubiquitination analyses were conducted to explore the interaction between TRIM24 and NLRP3. Subsequently, we found that TRIM24 negatively regulated NLRP3\/caspase\u20101\/IL\u20101\u03b2\u2010mediated pyroptosis and cell migration of hESC, and CY\u201009, the specific inhibitor of NLRP3, could reverse the promoted pyroptosis and cell migration induced by siTRIM24. Furthermore, TRIM24 interacted with NLRP3 and the upregulation of TRIM24 facilitated the ubiquitination of NLRP3 in ectopic hESC. Our findings suggest that TRIM24 may participate in the progression of endometriosis through the NLRP3\/caspase\u20101\/IL\u20101\u03b2\u2010mediated pyroptotic pathway via ubiquitination of NLRP3, which reveals the significant molecular mechanism underlying endometriosis.<\/jats:p>","DOI":"10.1002\/cbin.11592","type":"journal-article","created":{"date-parts":[[2021,3,16]],"date-time":"2021-03-16T10:57:49Z","timestamp":1615892269000},"page":"1561-1570","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":73,"title":["E3 ubiquitin ligase TRIM24 deficiency promotes NLRP3\/caspase\u20101\/IL\u20101\u03b2\u2010mediated pyroptosis in endometriosis"],"prefix":"10.1002","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1521-5974","authenticated-orcid":false,"given":"Yuanyuan","family":"Hang","sequence":"first","affiliation":[{"name":"Department of Traditional Chinese Medicine, Shanghai General Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine, Shanghai General Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoli","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine, Shanghai General Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuli","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai University of Traditional Chinese Medicine  Shanghai 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