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However, the role of miR\u2010100\u20105p in cyclic strain\u2010induced chondrocyte damage remains to be elucidated. miR\u2010100\u20105p and NADPH oxidase 4 (NOX4) were silenced or overexpressed in human primary articular chondrocytes. PKH\u201067 Dye was used to trace exosome endocytosis. Reactive oxygen species (ROS) production was monitored using DCFH\u2010DA. Cell apoptosis was measured using a flow cytometer. Quantitative RT\u2010PCR and Western blots were used to evaluate gene expression. Cyclic strain promoted ROS production and apoptosis in primary articular chondrocytes in a time\u2010dependent manner. HucMSCs\u2010derived exosomal miR\u2010100\u20105p inhibited cyclic strain\u2010induced ROS production and apoptosis in primary articular chondrocytes. miR\u2010100\u20105p directly targeted NOX4. Overexpressing NOX4 attenuated hucMSCs\u2010derived exosomes\u2010mediated protective effects in primary articular chondrocytes. Cyclic strain promotes ROS production and apoptosis in primary articular chondrocytes, which was abolished by hucMSCs\u2010derived exosomal miR\u2010100\u20105p through its target NOX4. The findings highlight the importance of miR\u2010100\u20105p\/NOX4 axis in primary articular chondrocytes injury and provide new insights into therapeutic strategies for articular chondrocytes injury and osteoarthritis.<\/jats:p>","DOI":"10.1002\/cbin.11657","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T04:18:43Z","timestamp":1625199523000},"page":"2096-2106","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":70,"title":["Exosomes from human umbilical cord mesenchymal stem cells inhibit ROS production and cell apoptosis in human articular chondrocytes via the miR\u2010100\u20105p\/NOX4 axis"],"prefix":"10.1002","volume":"45","author":[{"given":"Xiang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Joint Surgery, Changzheng Hospital Naval Medical University  Shanghai 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