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This study aimed to determine the function of M1M\u03c6\u2010exosomes (Exos) in the development of osteoporosis (OP) and the involvement of microRNA (miR)\u201098 and dual specificity phosphatase 1 (DUSP1). A murine model of OP was established using ovariectomies (OVX). Bone loss was observed in OVX\u2010treated mice, as manifested by reduced bone mineral density and decreased number of bone trabecula. The bone loss was further aggravated by treatment with M1M\u03c6\u2010Exos. Exos also suppressed osteogenic differentiation of MC3T3\u2010E1 cells. miRNA microarray analysis revealed that the miR\u201098 level was notably upregulated in cells after Exo treatment, and DUSP1 was confirmed as a target of miR\u201098. Meanwhile, downregulation of miR\u201098 or upregulation of DUSP1 restored the osteogenic differentiation ability of MC3T3\u2010E1 cells. In addition, upregulation of DUSP1 reduced bone loss in murine bone tissues and suppressed JNK phosphorylation. In summary, M1M\u03c6\u2010derived exosomal miR\u201098 exacerbates bone loss and OP by downregulating DUSP1 and activating the JNK signaling pathway. miR\u201098 may therefore serve as a therapeutic target in OP management.<\/jats:p>","DOI":"10.1002\/cbin.11690","type":"journal-article","created":{"date-parts":[[2021,8,25]],"date-time":"2021-08-25T08:34:42Z","timestamp":1629880482000},"page":"2452-2463","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["M1 macrophage\u2010derived exosomes aggravate bone loss in postmenopausal osteoporosis via a microRNA\u201098\/DUSP1\/JNK axis"],"prefix":"10.1002","volume":"45","author":[{"given":"Long","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Spine Surgery The 8th Medical Center of Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The 8th Medical Center of Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The 8th Medical Center of Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da","family":"Bao","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The 8th Medical Center of Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanpeng","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The 8th Medical Center of Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The 8th Medical Center of Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Litao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The 8th Medical Center of Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The 8th Medical Center of Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunfeng","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The 8th Medical Center of Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1887-3304","authenticated-orcid":false,"given":"Xiaobo","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The 8th Medical Center of Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanzheng","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Spine Surgery The 8th Medical Center of Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,8,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1530\/EJE-18-0056"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-015-0417-7"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.01255"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbspin.2018.02.009"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2017.02.007"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.3364"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-8587(17)30184-5"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.37088"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4933-2-14"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2020.115760"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20130538"},{"key":"e_1_2_8_13_1","first-page":"6221","article-title":"Bone marrow stem cells\u2010derived exosomes extracted from osteoporosis patients inhibit osteogenesis via microRNA\u201021\/SMAD7","volume":"22","author":"Jiang L. 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