{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:36:08Z","timestamp":1786977368483,"version":"build-2736575974"},"reference-count":49,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T00:00:00Z","timestamp":1632700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007432","name":"Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences","doi-asserted-by":"publisher","award":["ICR100\u2010500 and ICR100\u2010504"],"award-info":[{"award-number":["ICR100\u2010500 and ICR100\u2010504"]}],"id":[{"id":"10.13039\/501100007432","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004320","name":"Shiraz University of Medical Sciences","doi-asserted-by":"publisher","award":["96\u201001\u201074\u201014117"],"award-info":[{"award-number":["96\u201001\u201074\u201014117"]}],"id":[{"id":"10.13039\/501100004320","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Indoleamine 2, 3\u2010dioxygenase (IDO) catabolizes tryptophan, mediates immunomodulatory functions, and is released by stromal cells such as mesenchymal stem cells. The aims of this study were to investigate the effects of IDO silencing on immunosuppressive function of adipose\u2010derived mesenchymal stem cells (ASCs), T cells phenotype, and the proliferation\/migration of tumor cells. ASCs isolated from adipose tissues of healthy women were transfected with IDO\u2010siRNA. Galectin\u20103, transforming growth factor\u2010\u03b21, hepatocyte growth factor, and interleukin\u201010 as immunomodulators were measured in ASCs using qRT\u2010PCR. T cells phenotype, interferon\u2010\u03b3, and interleukin\u201017 expression were evaluated in peripheral blood lymphocytes (PBLs) cocultured with IDO silenced\u2010ASCs by flow cytometry and qRT\u2010PCR, respectively. Scratch assay was applied to assess the proliferation\/migration of MDA\u2010MB\u2010231 cell line. Galectin\u20103 was upregulated (\n                    <jats:italic>p\u2009<\/jats:italic>\n                    \u02c2\u20090.05) while hepatocyte growth factor was downregulated (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u02c2\u20090.05) in IDO\u2010silenced ASCs compared to control groups. Regulatory T cells were inhibited in PBLs cocultured with IDO\u2010silenced ASCs; also T helper2 was decreased in PBLs cocultured with IDO\u2010silenced ASCs relative to the scramble group. IDO\u2010silenced ASCs caused interferon\u2010\u03b3 overexpression but interleukin\u201017 downregulation in PBLs. The proliferation\/migration of MDA\u2010MB\u2010231 was suppressed after exposing to condition media of IDO\u2010silenced ASCs compared with condition media of untransfected (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01) and scramble\u2010transfected ASCs (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). The results exhibited the weakened capacity of IDO\u2010silenced ASCs for suppressing the immune cells and promoting the tumor cells' proliferation\/migration. IDO suppression may be utilized as a strategy for cancer treatment. Simultaneous blocking of immunomodulators along with IDO inhibitors may show more effects on boosting the efficiency of immune\u2010based cancer therapies.\n                  <\/jats:p>","DOI":"10.1002\/cbin.11698","type":"journal-article","created":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T05:07:00Z","timestamp":1631164020000},"page":"2544-2556","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effects of indoleamine 2, 3\u2010dioxygenase (IDO) silencing on immunomodulatory function and cancer\u2010promoting characteristic of adipose\u2010derived mesenchymal stem cells (ASCs)"],"prefix":"10.1002","volume":"45","author":[{"given":"Fahimeh","family":"Heidari","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine, School of Advanced Medical Sciences and Technologies Shiraz University of Medical Sciences Shiraz 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Technologies Shiraz University of Medical Sciences Shiraz Iran"},{"name":"School of Medicine, Shiraz Institute for Cancer Research Shiraz University of Medical Sciences Shiraz Iran"},{"name":"Department of Immunology, School of Medicine Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,27]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"597","article-title":"Indoleamine 2, 3\u2010dioxygenase is dispensable for the immunomodulatory function of stem cells from human exfoliated deciduous teeth","volume":"18","author":"Alipour R.","year":"2017","journal-title":"Cell Journal 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