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However, there is a lack of reports on the role of exosomal cirRNAs in the plasma of patients with AS. We isolated exosomes from the plasma of patients with AS and healthy individuals (controls). Subsequently, we investigated the circRNA profiles of the exosomes via high\u2010throughput RNA sequencing and identified 56 differentially expressed circRNAs in the exosomes of patients with AS compared with those of the healthy controls. Gene Ontology demonstrated that the differentially expressed circRNAs were mainly involved in the negative regulation of the activity of the transcription factor nuclear factor\u2010\u03baB and bone remodelling that is potentially related to AS. Kyoto Encyclopedia Genes and Genome\u00a0demonstrated that the most highly AS\u2010correlated pathways that were identified were \u2018notch signalling pathway\u2019 and those primarily involved with \u2018cholinergic synapse\u2019. Finally, we validated five differentially expressed circRNAs using quantitative real\u2010time polymerase chain reaction\u00a0and predicted their potential functions through the circRNA\u2013miRNA\u2013mRNA network. Our study is the first to report changes in exosomal circRNAs from plasma samples of patients with AS, and provide\u00a0novel targets for further investigation of molecular mechanisms and potential intervention therapy targets of AS.<\/jats:p>","DOI":"10.1002\/cbin.11760","type":"journal-article","created":{"date-parts":[[2022,1,6]],"date-time":"2022-01-06T12:14:00Z","timestamp":1641471240000},"page":"649-659","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Differential expression of circular RNAs in plasma exosomes from patients with ankylosing spondylitis"],"prefix":"10.1002","volume":"46","author":[{"given":"Lele","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Medicine, The Second Affiliated Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liyan","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine, The Second Affiliated Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yurong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine, The Second Affiliated Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhijiang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine, The Second Affiliated Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6654-687X","authenticated-orcid":false,"given":"Huqiang","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine, The Second Affiliated Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,1,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-016-3403-x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.100469"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.spinee.2018.06.349"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201702153"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2014.130"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1294"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep37982"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.21299"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-13-1426-1_9"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/826316"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-017-0043-5"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs20190286"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.39464"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8080909"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11993"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2667"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1053\/berh.2002.0243"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/7165893"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/gene.2017.11"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2959"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep22572"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.5114\/aoms.2019.84690"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3906\/sag-1304-140"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2015.10.013"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-014-0489-6"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.23512"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2020.04.010"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(16)30626-X"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2015-207490"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.28201"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-018-1618-8"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-019-1041-z"},{"key":"e_1_2_8_34_1","unstructured":"Wenker K. 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