{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T08:43:40Z","timestamp":1773391420609,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T00:00:00Z","timestamp":1648512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81770486"],"award-info":[{"award-number":["81770486"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81300200"],"award-info":[{"award-number":["81300200"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170268"],"award-info":[{"award-number":["82170268"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>High glucose (HG)\u2010induced dysfunction of vascular endothelial cells plays a crucial role in the development of diabetic vascular complications. Inhibition of cystathionine \u03b3\u2010synthase\/hydrogen sulfide (CSE\/H<jats:sub>2<\/jats:sub>S) pathway is one of the causes of vascular endothelial cell\u00a0injury induced by HG. Dopamine D1 receptors (DR1) are widely expressed and regulate important physiological functions in the vascular system. However, the effect of DR1 inhibition on HG\u2010induced vascular endothelial apoptosis by regulating the CSE\/H<jats:sub>2<\/jats:sub>S pathway is unclear. Therefore, we aimed to determine if DR1 can regulate the CSE\/H<jats:sub>2<\/jats:sub>S pathway and regulate the effect of DR1 on HG\u2010induced apoptosis in human umbilical vein endothelial cells. In this study, we found that HG treatment significantly decreased the expression of DR1 and CSE and the endogenous content of H<jats:sub>2<\/jats:sub>S;\u00a0DR1 agonist SKF 38393 reversed these effects, while sodium hydrosulfide\u00a0(NaHS) only increased CSE expression and the endogenous H<jats:sub>2<\/jats:sub>S production and had no effect on DR1 expression. Meanwhile, HG significantly increased\u00a0the intracellular calcium concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>),\u00a0and SKF 38393 further increased HG\u2010induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>. In addition, HG increased the lactate dehydrogenase activity, malondialdehyde and reactive oxygen species contents, apoptotic rate, the expression of cleaved caspase\u20103, caspase\u20109, and cytochrome <jats:italic>c<\/jats:italic>, and the activity of phosphorylated\u2010inhibitor of nuclear factor\u2010kappaB\u03b1 (NF\u2010\u03baB\u03b1) (p\u2010I\u03baB\u03b1) and phosphorylated\u2010NF\u2010\u03baB (p\u2010NF\u2010\u03baB), and reduced cell viability, superoxide dismutase activity, and Bcl\u20102 expressions. SKF 38393 and NaHS markedly reversed the effect of HG. The effect of SKF 38393 was similar to <jats:italic>N<\/jats:italic>\u2010acetyl\u2010\n<jats:sc>l<\/jats:sc>\u2010cysteine (an inhibitor of oxidative stress) or pyrrolidinedithiocarbamate ammonium (an NF\u2010kB inhibitor). Taken together, DR1 upregulates the CSE\/H<jats:sub>2<\/jats:sub>S pathway by increasing the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>, which inhibits HG\u2010induced apoptosis via downregulating NF\u2010\u03baB\/I\u03baB\u03b1 pathway in vascular endothelial cells.<\/jats:p>","DOI":"10.1002\/cbin.11794","type":"journal-article","created":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T11:28:48Z","timestamp":1647430128000},"page":"1098-1108","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Dopamine 1 receptors inhibit apoptosis via activating CSE\/H<sub>2<\/sub>S pathway in high glucose\u2010induced vascular endothelial cells"],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4164-9829","authenticated-orcid":false,"given":"Hongzhu","family":"Li","sequence":"first","affiliation":[{"name":"School of Medicine Xiamen University  Xiamen Fujian China"},{"name":"Department of Pathophysiology Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuxin","family":"Xi","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongxia","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guiquan","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengqi","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Can","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijie","family":"Jiao","sequence":"additional","affiliation":[{"name":"School of Medicine Xiamen University  Xiamen Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gensheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine, Second Affiliated Hospital Zhejiang University School of Medicine  Hangzhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Altaany","family":"Zaid","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Yarmouk University  Irbid Jordan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinghui","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,3,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc13-S067"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2010.12.008"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.14.1.649"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.110.002642"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22001-w"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-017-4331-2"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2020.05.015"},{"issue":"1","key":"e_1_2_9_9_1","first-page":"45","article-title":"Dopamine receptors and the cardiovascular system","volume":"57","author":"Clark B. J.","year":"1981","journal-title":"Postgraduate Medical Journal"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/1479164116666762"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2020.07.005"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00045.2011"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00473.2016"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2019.101356"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2016.05.010"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12933-018-0763-3"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1423-0127-18-18"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2007.00177.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S242521"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2017.3330"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.26402\/jpp.2019.6.01"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2013.12.216"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1998.78.1.189"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/wsbm.1331"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201901304R"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/792393"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2014.10.037"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/2047487319878373"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2020.111849"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/1753-0407.12521"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051874"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.01568"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201300841"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10528-013-9581-1"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00017.2011"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1162667"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40659-018-0157-8"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.11794","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbin.11794","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.11794","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T08:01:56Z","timestamp":1692691316000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.11794"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,29]]},"references-count":36,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["10.1002\/cbin.11794"],"URL":"https:\/\/doi.org\/10.1002\/cbin.11794","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,29]]},"assertion":[{"value":"2021-09-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}