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It is one of the most studied <jats:italic>Leishmania<\/jats:italic> species not only during vector interaction but also in the vertebrate host. Lipophosphoglycan (LPG) is the <jats:italic>Leishmania<\/jats:italic> multifunctional virulence factor during host\u2013parasite interaction, whose polymorphisms are involved in the immunopathology of leishmaniasis. Although natural hybrids occur in nature, hybridization of <jats:italic>L. major<\/jats:italic> strains in the laboratory was successfully demonstrated. However, LPG expression in the hybrids remains unknown. LPGs from parental (Friedlin, Fn and Seidman,\u00a0Sd) and hybrids (FnSd3, FnSd4A, FnSd4B, and FnSd6F) were extracted, purified, and their repeat units analyzed by immunoblotting and fluorophore\u2010assisted carbohydrate electrophoresis. Parental strains have distinct profiles in LPG expression, and a mixed profile was observed for all hybrids. Variable levels of NO production by macrophages were detected after LPG exposure (parental and hybrids) and were strain specific.<\/jats:p>","DOI":"10.1002\/cbin.11798","type":"journal-article","created":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T12:21:14Z","timestamp":1647865274000},"page":"1169-1174","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of hybridization on lipophosphoglycan expression in <i>Leishmania major<\/i>"],"prefix":"10.1002","volume":"46","author":[{"given":"Karine R. 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