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The subretinal fibrosis subsequent to choroidal neovascularization (CNV) is an important feature in the late stage of wet AMD and is considered to be one reason for incomplete response to anti\u2010VEGF drugs. Recent studies have shown that pericyte\u2010myofibroblast transition (PMT) is an important pathological process involving fibrotic diseases of various organs. However, the specific role and mechanism of PMT in the subretinal fibrosis of CNV have not been clarified. It has been clear that the Hippo pathway along with its downstream effector Yes\u2010associated protein (YAP) plays an important role in both epithelial and endothelial myofibroblast development. Therefore, we speculate whether YAP participates in PMT of pericytes and promotes fibrosis of CNV. In this study, experimental CNV was induced by laser photocoagulation in C57BL\/6J (B6) mice, and aberrant YAP overexpression was detected in the retinal pigment epithelial\/choroid\/sclera tissues of the laser\u2010injured eyes. YAP knockdown reduced the proliferation, migration, and differentiation of human retinal microvascular pericytes in vitro. It also reduced subretinal fibrosis of laser\u2010induced CNV in vivo. Moreover, by proteomics\u2010based analysis of pericyte conditioned medium (PC\u2010CM) and bioinformatic analyses, we identified that the crosstalk between Hippo\/YAP and MAPK\/Erk was involved in expression of filamin A in hypoxic pericytes. These findings suggest that Hippo\/YAP and MAPK\/Erk are linked together to mediate pericyte proliferation, migration as well as differentiation, which may embody potential implications for treatment in diseases related to CNV.<\/jats:p>","DOI":"10.1002\/cbin.11809","type":"journal-article","created":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T09:09:36Z","timestamp":1651050576000},"page":"1249-1263","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Silencing of YAP attenuates pericyte\u2010myofibroblast transition and subretinal fibrosis in experimental model of choroidal neovascularization"],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3068-2666","authenticated-orcid":false,"given":"Ya\u2010hui","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Zhongshan Hospital Fudan University Shanghai China"},{"name":"Department of Ophthalmology Northern Jiangsu Peoples' Hospital Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010fan","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Zhongshan Hospital Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Zhongshan Hospital Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Zhongshan Hospital Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5240-3915","authenticated-orcid":false,"given":"Yuan\u2010zhi","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Zhongshan Hospital Fudan University Shanghai China"},{"name":"Department of Ophthalmology, Xiamen Branch, Zhongshan Hospital Fudan University Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,27]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Angiogenesis","author":"Adair T. 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