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CXCL13 is a chemokine from the CXC chemokine family, which has been shown to destroy the barrier function of umbilical vein endothelial cells with its receptor CXCR5. Here, we aimed to investigate the role of CXCL13\/CXCR5 signaling axis in BBB. The invasive ability of bEnd.3 cells was determined by the Transwell invasion assay. The barrier integrity of bEnd.3 cells was assessed by detecting trans\u2010endothelial electrical resistance, the permeability to fluorescein isothiocyanate\u2212dextran, and the expression levels of the tight junction protein E\u2010cadherin. Lipopolysaccharide (LPS)\u2010activated microglia promoted invasion and barrier dysfunction, and upregulated CXCR5 and p\u2010p38 expression levels in cocultured bEnd.3 cells. However, the effects of activated microglia were alleviated by knocking down CXCR5 in cocultured bEnd.3 cells. Furthermore, recombinant CXCL13 promoted invasion and barrier dysfunction, and upregulated the expression levels of p\u2010p38 in bEnd.3 cells; however, its effects were abolished by treating bEnd.3 cells with the p38 inhibitor SB203580. Our data tentatively demonstrated that LPS\u2010activated microglial cells may promote invasion and barrier dysfunction in bEnd.3 cells by regulating the CXCL13\/CXCR5 axis and p38 signaling.<\/jats:p>","DOI":"10.1002\/cbin.11832","type":"journal-article","created":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T09:18:49Z","timestamp":1654593529000},"page":"1510-1518","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Activated microglia promote invasion and barrier dysfunction of brain endothelial cells via regulating the CXCL13\/CXCR5 axis"],"prefix":"10.1002","volume":"46","author":[{"given":"Qiaolei","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Hematology Lishui People's Hospital, Lishui Zhejiang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Hematology Lishui People's Hospital, Lishui Zhejiang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghua","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hematology Lishui People's Hospital, Lishui Zhejiang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangli","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Hematology Lishui People's Hospital, Lishui Zhejiang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqiu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hematology Lishui People's Hospital, Lishui Zhejiang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7328-9632","authenticated-orcid":false,"given":"Bingmu","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Hematology Lishui People's Hospital, Lishui Zhejiang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,6,7]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/1352458512473362"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2018.09.019"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-020-01253-6"},{"key":"e_1_2_11_5_1","first-page":"55","article-title":"CXCL13 is involved in the Lipopolysaccharide\u2010Induced hyperpermeability of umbilical vein endothelial cells","volume":"24","author":"Chen W.","year":"2020","journal-title":"Age"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jcbfm.9600229"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22692"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21082927"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201902203RR"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.4172\/2155-9899.1000460"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI81950"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-017-0504-2"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00471"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1749-8104-7-27"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2017.04.014"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20030571"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-018-1374-3"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-017-0948-9"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2019.00521"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00371.2011"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2015.154"},{"issue":"1","key":"e_1_2_11_22_1","first-page":"167","article-title":"Oligodendrocyte precursor cells support blood\u2010brain barrier integrity via TGF\u2010beta signaling","volume":"39","author":"Seo J.","year":"2019","journal-title":"J Cereb Blood Flow Metab"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2018.00494"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12922"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-015-0298-7"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2018.11.005"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-2230-9"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114511002868"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.14963"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.302157"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000206281.77178.ac"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12854"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix233"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/13813455.2020.1784952"},{"key":"e_1_2_11_35_1","doi-asserted-by":"crossref","unstructured":"Zhao B. 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