{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:36:53Z","timestamp":1740105413187,"version":"3.37.3"},"reference-count":31,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T00:00:00Z","timestamp":1658793600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hematopoietic stem cells (HSCs) transplantation is considered a suitable treatment for malignant or nonmalignant hematological diseases. This study aims to investigate the HSCs homing factors in bone marrow (BM) donors of acute lymphoblastic leukemia (ALL) patients following granulocyte colony\u2010stimulating factor (G\u2010CSF) injection, as well as the G\u2010CSF effects on BM transplantation quality in these patients. To mobilize HSCs into peripheral blood, G\u2010CSF was used for ALL patient's BM donors. For HSCs counting, CD34+ cells were evaluated in analogous and autologous donors using flow cytometry. The expression of stem cell homing factors in CD34+ cells and peripheral blood mononuclear cells (PBMCs) were investigated using a real\u2010time\u00a0polymerase chain reaction. Finally, hematological factors after BM transplantation in ALL patients were assessed. According to our results, after G\u2010CSF injection, the level of CD34+\u00a0HSCs was statistically increased. Besides, autologous donors showed a higher level of CD34+\u00a0cells compared to analogous donors before and after G\u2010CSF injection. Additionally, a higher number of CD34+\u00a0HSCs was achieved in the autologous samples following G\u2010CSF injection. Furthermore, after G\u2010CSF injection, the expression of matrix metalloproteinase\u00a0(MMP)\u20102, MMP\u20109 was increased; while, stromal cell\u2010derived factor 1,\u00a0intercellular adhesion molecule\u20101,\u00a0and vascular\u00a0cell adhesion molecule\u20101 expression were decreased. Moreover, the expression of C\u2010X\u2010C chemokine receptor type 4, lymphocyte function\u2010associated antigen 1,\u00a0and very\u00a0late antigen\u20104 in CD34+\u00a0cells and PBMCs were decreased. BM transplantation on Day 90 also caused an increased level of white blood cells, red blood cells, and platelets as compared to the first day; however, no statistical differences were observed in hemoglobin level. In conclusion, G\u2010CSF by altering the expression of HSCs homing factors in ALL donors improves BM transplantation quality in ALL patients.<\/jats:p>","DOI":"10.1002\/cbin.11865","type":"journal-article","created":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T20:22:45Z","timestamp":1658866965000},"page":"1876-1885","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The altered expression of homing factors in CD34<sup>+<\/sup> hematopoietic stem cells following G\u2010CSF injection and its effects on transplantation quality in ALL patients"],"prefix":"10.1002","volume":"46","author":[{"given":"Seyede\u2010Leila","family":"Shafiei","sequence":"first","affiliation":[{"name":"Hematology and Oncology Research Center Tabriz University of Medical Sciences  Tabriz 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