{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T04:57:16Z","timestamp":1769749036935,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,8,21]],"date-time":"2022-08-21T00:00:00Z","timestamp":1661040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It has been reported that long noncoding RNA (lncRNA) KCNQ1 opposite strand\/antisense transcript 1 (KCNQ1OT1) played an important role in myocardial infarction (MI). However, the regulatory network behind KCNQ1OT1 in MI is largely unknown. Quantitative real time polymerase chain reaction (qRT\u2010PCR) was applied to detect the enrichment of KCNQ1OT1, microRNA\u2010130a\u20103p (miR\u2010130a\u20103p) and zinc finger 791 (ZNF791). The viability and apoptosis of AC16 cells were measured by (4,5\u2010Dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide (MTT) assay and flow cytometry. Enzyme\u2010linked immunosorbent assay (ELISA) was conducted to assess the inflammation and oxidative stress status of AC16 cells. The targeted relationship between miR\u2010130a\u20103p and KCNQ1OT1 or ZNF791 was predicted by StarBase bioinformatic database, and dual\u2010luciferase reporter assay and RNA immunoprecipitation (RIP) assay were carried out to verify these predictions. Hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) stimulation caused a significant upregulation in the expression of KCNQ1OT1, while the level of miR\u2010130a\u20103p showed an opposite phenomenon. KCNQ1OT1 was a crucial downstream component in H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010mediated toxic effects, and KCNQ1OT1 accelerated H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced toxic effects in AC16 cells. KCNQ1OT1 could sponge miR\u2010130a\u20103p and down\u2010regulate its expression. MiR\u2010130a\u20103p exerted opposite effects to KCNQ1OT1, and the depletion of miR\u2010130a\u20103p attenuated the protective effects of KCNQ1OT1 intervention on AC16 cells exposed to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. MiR\u2010130a\u20103p could bind to ZNF791, and ZNF791 served as the target of miR\u2010130a\u20103p to promote H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced injury of AC16 cells. ZNF791 was modulated by KCNQ1OT1\/miR\u2010130a\u20103p signaling in H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010treated AC16 cells. In all, lncRNA KCNQ1OT1 deteriorated H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010mediated injury in cardiomyocytes through upregulating ZNF791 via serving as a molecular sponge for miR\u2010130a\u20103p.<\/jats:p>","DOI":"10.1002\/cbin.11873","type":"journal-article","created":{"date-parts":[[2022,8,22]],"date-time":"2022-08-22T05:30:04Z","timestamp":1661146204000},"page":"2018-2027","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["LncRNA KCNQ1OT1 contributes to hydrogen peroxide\u2010induced apoptosis, inflammation, and oxidative stress of cardiomyocytes via miR\u2010130a\u20103p\/ZNF791 axis"],"prefix":"10.1002","volume":"46","author":[{"given":"Hong","family":"Xin","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Medicine The First Affiliated Hospital of Xi 'an Medical University  Xi 'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengliang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of General Practice The First Affiliated Hospital of Xi 'an Medical University  Xi 'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianzhi","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine The First Affiliated Hospital of Xi 'an Medical University  Xi 'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinlong","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine The First Affiliated Hospital of Xi 'an Medical University  Xi 'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5333-3229","authenticated-orcid":false,"given":"Meixue","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine The First Affiliated Hospital of Xi 'an Medical University  Xi 'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/4.4.685"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.26175"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(92)90372-Y"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.06.122"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.114.001249"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.48.1.1"},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","unstructured":"Li C. Miao R. Zhang J. Qu K. &Liu C.(2018).Long non\u2010coding RNA KCNQ1OT1 mediates the growth of hepatocellular carcinoma by functioning as a competing endogenous RNA of miR\u2010504.International Journal of Oncology.","DOI":"10.3892\/ijo.2018.4313"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S188054"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02280-y"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2015.10.011"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.4254\/wjh.v9.i23.1001"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2014.5837"},{"key":"e_1_2_9_14_1","first-page":"3088","article-title":"MiR\u201024 alleviates cardiomyocyte apoptosis after myocardial infarction via targeting BIM","volume":"21","author":"Pan L. 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