{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T07:48:16Z","timestamp":1747986496562,"version":"3.37.3"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T00:00:00Z","timestamp":1661817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Reprimo (RPRM), a target gene of p53, is a known tumor suppressor. DNA damage induces RPRM, which triggers p53\u2010dependent G2 arrest by inhibiting cyclin B1\/Cdc2 complex activation and promotes DNA damage\u2010induced apoptosis. RPRM negatively regulates ataxia\u2010telangiectasia mutated by promoting its nuclear\u2010cytoplasmic translocation and degradation, thus inhibiting DNA damage. Therefore, RPRM plays a crucial role in DNA damage response. Moreover, the loss of RPRM confers radioresistance in mice, which enables longer survival and less severe intestinal injury after radiation exposure. However, the role of RPRM in radiation\u2010induced hematopoietic system injury remains unknown. Herein, utilizing a RPRM\u2010knockout mouse model, we found that RPRM deletion did not affect steady\u2010state hematopoiesis in mice. However, RPRM knockout significantly alleviated radiation\u2010induced hematopoietic system injury and preserved mouse hematopoietic regeneration in hematopoietic stem cells (HSCs) against radiation\u2010induced DNA damage. Further mechanistic studies showed that RPRM loss significantly increased EGFR expression and phosphorylation in HSCs to activate STAT3 and DNA\u2010PKcs, thus promoting HSC DNA repair and proliferation. These findings reveal the critical role of RPRM in radiation\u2010induced hematopoietic system injury, confirming our hypothesis that RPRM may serve as a novel target for radiation protection.<\/jats:p>","DOI":"10.1002\/cbin.11900","type":"journal-article","created":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T20:55:03Z","timestamp":1661892903000},"page":"2158-2172","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["RPRM deletion preserves hematopoietic regeneration by promoting EGFR\u2010dependent DNA repair and hematopoietic stem cell proliferation post ionizing radiation"],"prefix":"10.1002","volume":"46","author":[{"given":"Zixuan","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Radiation Medicine and Protection Soochow University Suzhou Jiangsu China"},{"name":"School of Radiation Medicine and Protection, Suzhou Medical College of Soochow University\/Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions Soochow University Suzhou Jiangsu China"},{"name":"Cyrus Tang Medical Institute, Collaborative Innovation Center of Hematology Soochow University Suzhou Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhou","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Radiation Medicine and Protection Soochow University Suzhou Jiangsu China"},{"name":"School of Radiation Medicine and Protection, Suzhou Medical College of Soochow University\/Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions Soochow University Suzhou Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuaiyu","family":"Tian","sequence":"additional","affiliation":[{"name":"Cyrus Tang Medical Institute, Collaborative Innovation Center of Hematology Soochow University Suzhou Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kailu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Cyrus Tang Medical Institute, Collaborative Innovation Center of Hematology Soochow University Suzhou Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gangli","family":"An","sequence":"additional","affiliation":[{"name":"Cyrus Tang Medical Institute, Collaborative Innovation Center of Hematology Soochow University Suzhou Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yarui","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Radiation Medicine and Protection Soochow University Suzhou Jiangsu China"},{"name":"School of Radiation Medicine and Protection, Suzhou Medical College of Soochow University\/Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions Soochow University Suzhou Jiangsu 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