{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T12:54:50Z","timestamp":1782564890577,"version":"3.54.5"},"reference-count":44,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,9,18]],"date-time":"2022-09-18T00:00:00Z","timestamp":1663459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Exosomes (Exo) originated from bone marrow mesenchymal stem cells (BMSCs) have therapeutic impacts on osteonecrosis of the femoral head (ONFH), and microRNA (miR)\u2010532\u20105p has been confirmed to participate in ONFH progression. In the research, it was figured out whether BMSCs\u2010Exo could relieve ONFH by delivering miR\u2010532\u20105p. MG\u201063 cells were treated with DEX to construct an ONFH cell model in vitro. The effects of Exo and miR\u2010532\u20105p on the cell viability, lactate dehydrogenase (LDH) content, and apoptosis of BMSCs were detected. The ONFH rat model was established, and the effect of BMSCs\u2010Exo delivering miR\u2010532\u20105p on the pathological damage of ONFH rats was evaluated. Changes in nuclear receptor coactivator\u20103 (NCOA3) and apoptotic proteins were assessed by western blot. The relationship between miR\u2010532\u20105p and NCOA3 was verified by dual luciferase reporter experiments. miR\u2010532\u20105p was elevated in vivo and in vitro ONFH\u2010models, while NCOA3 expression was reduced. Overexpression of miR\u2010532\u20105p aggravated DEX toxicity in osteoblasts, decreased cell viability, and promoted apoptosis. Knockdown of miR\u2010532\u20105p made Exo further attenuate the toxic effect of DEX on osteoblasts and inhibited apoptosis. The protective effect of miR\u2010532\u20105p\u2010delivering Exo on osteoblasts was reversed by NCOA3 silencing. In addition, in vivo experiments also confirmed that knockdown of miR\u2010532\u20105p enhanced the therapeutic effect of Exo on ONFH rats. This study demonstrates that miR\u2010532\u20105p\u2010delivering BMSCs\u2010Exo inhibits osteoblast viability and promote apoptosis by targeting NCOA3, thereby aggravating ONFH development.<\/jats:p>","DOI":"10.1002\/cbin.11902","type":"journal-article","created":{"date-parts":[[2022,9,18]],"date-time":"2022-09-18T11:53:50Z","timestamp":1663502030000},"page":"2185-2197","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Bone marrow mesenchymal stem cells\u2010derived exosomes mediate nuclear receptor coactivator\u20103 expression in osteoblasts by delivering miR\u2010532\u20105p to influence osteonecrosis of the femoral head development"],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0802-2509","authenticated-orcid":false,"given":"XiaoYong","family":"Lan","sequence":"first","affiliation":[{"name":"Department of Rehabilitation Medicine Second Affiliated Hospital of Nanchang 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