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It has been reported that miR\u2010322 is involved in MIRI progression, while the molecular mechanism of miR\u2010322 in regulating MIRI progression needs to be further probed. MIRI cell model was established by oxygen\u2010glucose deprivation\/reoxygenation (OGD\/R). Cell viability was assessed using MTS assay. Flow cytometry and terminal deoxynucleotidyl transferase\u2010mediated dUTP nick\u2010end labeling staining were employed to analyze cell apoptosis. In addition, the interactions between miR\u2010322, Smad7\/Smurf2, hypoxia\u2010inducible factor alpha (HIF\u20101\u03b1), and \u03b2\u2010catenin were verified by dual\u2010luciferase reporter gene assay. Our results displayed that miR\u2010322 was significantly downregulated in OGD\/R\u2010treated H9c2 cells, and its overexpression resulted in increased cell viability and reduced the apoptosis. Smurf2 and Smad7 were identified as the direct targets of miR\u2010322. Smad7 knockdown or Smurf2 knockdown increased OGD\/R\u2010treated H9c2 cell viability and suppressed the apoptosis. Meanwhile, miR\u2010322 mimics abolished the mitigating effect of Smad7 or Smurf2 overexpression on MIRI. In addition, the Smad3\/\u03b2\u2010catenin pathway was identified as the downstream pathway of Smurf2\/Smad7. Moreover, it was found that HIF\u20101\u03b1 interacted with the miR\u2010322 promoter, and \u03b2\u2010catenin interacted with the HIF\u20101\u03b1 promoter to form a loop. HIF\u20101\u03b1\u2010induced upregulated miR\u2010322 activated the Smad3\/\u03b2\u2010catenin pathway by targeting Smurf2 and Smad7 to improve MIRI; meanwhile, \u03b2\u2010catenin\/HIF\u20101\u03b1 formed a positive feedback loop to continuously improve MIRI.<\/jats:p>","DOI":"10.1002\/cbin.11954","type":"journal-article","created":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T07:22:52Z","timestamp":1679556172000},"page":"894-906","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["HIF\u20101\u03b1\u2010induced upregulated miR\u2010322 forms a feedback loop by targeting Smurf2 and Smad7 to activate Smad3\/\u03b2\u2010catenin\/HIF\u20101\u03b1, thereby improving myocardial ischemia\u2010reperfusion injury"],"prefix":"10.1002","volume":"47","author":[{"given":"Wei","family":"Dong","sequence":"first","affiliation":[{"name":"Department of Cardiology The First Affiliated Hospital of Nanchang University Nanchang 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