{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T09:00:46Z","timestamp":1765357246264,"version":"3.40.5"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T00:00:00Z","timestamp":1670544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82260031"],"award-info":[{"award-number":["82260031"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Longitudinal investigations have revealed the unique attributes of mesenchymal stem\/stromal cells (MSCs) in regenerative medicine. However, the spatio\u2010temporal metabolokinetics and efficacy of MSCs with vascular cell adhesion molecule 1 (also known as CD106) expression in phenotypes and therapeutic effect upon acute lung injury (ALI) mice are largely obscure. For the purpose, we took advantage of the \u201c3IL\u201d\u2010based strategy and Lentivirus\u2010mediated green fluorescent protein (GFP) delivery for the generation of the CD106<jats:sup>+<\/jats:sup> subset (denote as CD106<jats:sup>+<\/jats:sup>\u2010MSCs) from umbilical cord\u2010derived MSCs (denote as NT\u2010MSCs). Therewith, the cellular phenotypes of CD106<jats:sup>+<\/jats:sup>\u2010MSCs including immunophenotypes, multilineage differentiation potential towards adipocytes and osteoblasts were confirmed by flow cytometry and qRT\u2010PCR assay. Meanwhile, multifaceted characteristics of transcriptomic features were analyzed by utilizing the RNA\u2010SEQ and bioinformatics. Furthermore, to compare the therapeutic effects and spatio\u2010temporal dynamics of CD106<jats:sup>+<\/jats:sup>\u2010MSCs, we conducted in vivo fluorescent tracer, hematoxylin and eosin staining, blood smear, blood routine and cytokine detection in mice. Herein, we generated CD106<jats:sup>+<\/jats:sup>\u2010MSCs with GFP expression and confirmed the conservative property of phenotypes. Compared to NT\u2010MSCs with minimal CD106 expression, CD106<jats:sup>+<\/jats:sup>\u2010MSCs manifested consistent distribution and metabolokinetics in vivo but with preferable ameliorative effect upon the pathological appearance and proinflammatory cytokine secretion in ALI mice. Collectively, our data indicated the preferable therapeutic effects of CD106<jats:sup>+<\/jats:sup>\u2010MSCs upon ALI mice, which would benefit the further exploration of the CD106<jats:sup>+<\/jats:sup> subset for pulmonary diseases and investigational new drug application purposes.<\/jats:p>","DOI":"10.1002\/cbin.11976","type":"journal-article","created":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T17:54:45Z","timestamp":1670608485000},"page":"720-730","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Spatio\u2010temporal metabolokinetics and therapeutic effect of CD106<sup>+<\/sup> mesenchymal stem\/stromal cells upon mice with acute lung injury"],"prefix":"10.1002","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6540-0943","authenticated-orcid":false,"given":"Leisheng","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Medicine Nankai University  Tianjin China"},{"name":"Key Laboratory of Molecular Diagnostics and Precision Medicine for Surgical Oncology in Gansu Province &amp; NHC Key Laboratory of Diagnosis and Therapy of Gastrointestinal Tumor Gansu Provincial Hospital  Lanzhou China"},{"name":"Center for Cellular Therapies The First Affiliated Hospital of Shandong First Medical University  Ji\u2010nan China"},{"name":"Key Laboratory of Radiation Technology and Biophysics, Hefei Institute of Physical Science Chinese Academy of Sciences  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Zhuo","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The First Affiliated Hospital of Fujian Medical University  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Medicine Nankai University  Tianjin China"},{"name":"National Engineering Research Center of Cell Products AmCellGene Engineering Co., Ltd  Tianjin China"},{"name":"Tianjin Key Laboratory of Engineering Technologies for Cell Pharmaceutical  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,12,9]]},"reference":[{"issue":"1","key":"e_1_2_11_2_1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.2174\/1574888X16666210712212421","article-title":"Visualized analyses of investigations upon mesenchymal stem\/stromal cell\u2010based cytotherapy and underlying mechanisms for COVID\u201019 associated ARDS","volume":"17","author":"Aitong W.","year":"2021","journal-title":"Current Stem Cell Research & 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