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However, the effect of ASPM in anaplastic thyroid carcinoma (ATC) has not yet been understood. The present study is to elucidate the function of ASPM in the migration and invasion of ATC. ASPM expression is incrementally upregulated in ATC tissues and cell lines. Knockout (KO) of ASPM pronouncedly attenuates the migration and invasion of ATC cells. ASPM KO significantly reduces the transcript levels of Vimentin, N\u2010cadherin, and Snail and increases E\u2010cadherin and Occludin, thereby inhibiting epithelial\u2010to\u2010mesenchymal transition (EMT). Mechanistically, ASPM regulates the movement of ATC cells by inhibiting the ubiquitin degradation of KIF11 and thus stabilizing it via direct binding to it. Moreover, xenograft tumors in nude mice proved that KO of ASPM could ameliorate tumorigenesis and tumor growth accompanied by a decreased protein expression of KIF11 and an inhibition of EMT. In conclusion, ASPM is a potentially useful therapeutic target for ATC. Our results also reveal a novel mechanism by which ASPM inhibits the ubiquitin process in KIF11.<\/jats:p>","DOI":"10.1002\/cbin.12012","type":"journal-article","created":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T14:39:56Z","timestamp":1678286396000},"page":"1209-1221","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["ASPM promotes migration and invasion of anaplastic thyroid carcinoma by stabilizing KIF11"],"prefix":"10.1002","volume":"47","author":[{"given":"Qilu","family":"Fang","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Key Laboratory of Head and Neck Translational Research of Zhejiang Province, The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC) Chinese Academy of Sciences Hangzhou 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